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. 2019 Feb;56(2):1078-1083.
doi: 10.1007/s13197-018-03556-x. Epub 2019 Feb 4.

Incidence and antimicrobial susceptibility of Staphylococcus aureus isolated from ready-to-eat foods of animal origin from tourist destinations of North-western Himalayas, Himachal Pradesh, India

Priyanka Lakhanpal  1 , Ashok Kumar Panda  1 , Rajesh Chahota  2 , Shivani Choudhary  1 , Sidharath Dev Thakur  1
Affiliations

Incidence and antimicrobial susceptibility of Staphylococcus aureus isolated from ready-to-eat foods of animal origin from tourist destinations of North-western Himalayas, Himachal Pradesh, India

Priyanka Lakhanpal et al. J Food Sci Technol. 2019 Feb.

Abstract

This study was aimed to determine the incidence of Staphylococcus aureus in ready-to-eat (RTE) milk (n = 120) and meat (n = 120) products from various tourist places in north western Himalayas, Himachal Pradesh, India. S. aureus isolates and its enterotoxins; A, B, D and E were characterized by conventional and molecular methods. Antimicrobial susceptibility (AMS) profiles of S. aureus isolates were determined by disk diffusion method using Clinical and Laboratory Standards Institute criteria. Overall, 6.7% (n = 16/240) food samples were positive for S. aureus. PCR amplification of nucA confirmed all biochemically characterized isolates as S. aureus. Incidence of S. aureus was higher (10.0%) in RTE milk products than meat products (3.3%). S. aureus contamination levels were highest in milk cake/khoa (26.0%, p = 0.0002) followed by ice cream/kulfi (10.0%, p = 0.4), mutton momo (10.0%, p = 0.4), burfi (3.3%, p = 0.7) and chicken momo (3.3%, p = 0.7). None of the isolates carried genes for S. aureus enterotoxins; A, B, D and E. AMS testing revealed seven different resistance patterns and 81.3% multi drug resistance. All the isolates were resistant to ampicillin. High resistance levels were observed against methicillin (93.7%), clindamycin (68.8%), erythromycin (56.3%) and vancomycin (43.8%). Vancomycin resistant (n = 7) isolates were also resistant to methicillin. All isolates were susceptible to novobiocin.

Keywords: Enterotoxin; Methicillin; Novobiocin; Vancomycin; nucA gene.

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Conflict of interest statement

Compliance with ethical standardsThere is no conflict of interests.

References

    1. Argudín MA, Tenhagen BA, Fetsch A, Sachsenröder J, Käsbohrer A, Schroeter A, Hammerl JA, Hertwig S, Helmuth R, Bräunig J, Mendoza MC, Appel B, Rodicio MR, Guerra B. Virulence and resistance determinants of German Staphylococcus aureus ST398 isolates from nonhuman sources. Appl Environ Microbiol. 2011;77:3052–3060. doi: 10.1128/AEM.02260-10. - DOI - PMC - PubMed
    1. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45:493–496. doi: 10.1093/ajcp/45.4_ts.493. - DOI - PubMed
    1. Bhatia A, Zahoor S. Staphylococcus aureus enterotoxins: a review. J Clin Diagn Res. 2007;1:188–197.
    1. Carfora V, Caprioli A, Marri N, Sagrafoli D, Boselli C, Giacinti G, Giangolini G, Sorbara L, Dottarelli S, Battisti A, Amatiste S. Enterotoxin genes, enterotoxin production, and methicillin resistance in Staphylococcus aureus isolated from milk and dairy products in central Italy. Int Dairy J. 2015;42:2–15. doi: 10.1016/j.idairyj.2014.10.009. - DOI
    1. CLSI (2013) Performance standards for antimicrobial Susceptibility Testing. Twenty-Third Informational Supplement. CLSI document M100-S23. Wayne, PA. Clinical and Laboratory Standards Institute

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