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. 2019 Jan-Mar;11(1):60-68.
doi: 10.4103/jpbs.JPBS_58_18.

Antioxidant Potential of Solanum nigrum Aqueous Leaves Extract in Modulating Restraint Stress-Induced Changes in Rat's Liver

Syed K Zaidi  1 , Shakeel A Ansari  1 , Shams Tabrez  2 , Muhammad I Naseer  1 , Moyad J Shahwan  3 , Naheed Banu  4 , Muhammad H Al-Qahtani  1
Affiliations

Antioxidant Potential of Solanum nigrum Aqueous Leaves Extract in Modulating Restraint Stress-Induced Changes in Rat's Liver

Syed K Zaidi et al. J Pharm Bioallied Sci. 2019 Jan-Mar.

Abstract

Introduction: This study was carried out to evaluate the antioxidant potential of crude extract of Solanum nigrum leaves and its active constituents as treatment against restraint stress in rat's liver.

Methods: For this purpose, male albino Wistar rats were treated with crude extract of leaves and its alkaloid and flavonoid fractions both before and after 6 h of acute restraint stress. Prooxidant status of rat liver was assessed by determining the levels of thiobarbituric acid reactive substances, reduced glutathione, alkaline phosphatase, alanine transaminase, aspartate aminotransferase, and the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST).

Results: Six hours of restraint stress generated oxidative stress in rat's liver resulted in a significant rise in the level of the aforementioned liver enzymes. On the other hand, SOD, CAT, and GST enzymatic activities showed a significant decline in their level. The administration of crude leaves extract, both before and after stress exposure, significantly prevented the rise in the level of liver enzymes and reverted the activities of studied biochemical parameters toward their normal control values. However, the reversion was found to be more prominent in after-stress group.

Conclusion: The aforementioned results highlight the significant antioxidant potential of S. nigrum extracts. On the basis of our study, we suggest the possible use of S. nigrum leaves extract as a nutritional supplement for combating oxidative stress induced damage.

Keywords: Antioxidant enzymes; Solanum nigrum; liver; oxidative stress.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Effect of crude Solanum nigrum leaves extract treatment on immobilization stress-induced changes in liver’s superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione (GSH). Significant decrease in antioxidant enzymes activities and glutathione level were observed after immobilization stress. The pre- and post-stress extract treatment revert the deranged free radical system toward their normal values with a relative dominance by latter. (*P values compared with controls, #P values compared with stressed rats, where * <0.05 and # <0.05)
Figure 2
Figure 2
Effect of crude extract of Solanum nigrum leaves on restraint stress-induced changes in liver’s glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP), and malondialdehyde (MDA). Significant increase in liver markers enzymes along with a significant increase in MDA levels were observed after restraint stress. The pre- and post-stress extract treatment revert the deranged free radical system toward their normal values with a relative dominance by latter. (*P values compared with controls, #P values compared with stressed rats, where * <0.05 and # <0.05)
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