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. 2019 Feb 17:2019:5620989.
doi: 10.1155/2019/5620989. eCollection 2019.

The Green Synthesis of MgO Nano-Flowers Using Rosmarinus officinalis L. (Rosemary) and the Antibacterial Activities against Xanthomonas oryzae pv. oryzae

Yasmine Abdallah #  1   2 , Solabomi Olaitan Ogunyemi #  1 , Amro Abdelazez  3 , Muchen Zhang  1 , Xianxian Hong  1 , Ezzeldin Ibrahim  1   4 , Afsana Hossain  1 , Hatem Fouad  1   5 , Bin Li  1 , Jianping Chen  6
Affiliations

The Green Synthesis of MgO Nano-Flowers Using Rosmarinus officinalis L. (Rosemary) and the Antibacterial Activities against Xanthomonas oryzae pv. oryzae

Yasmine Abdallah et al. Biomed Res Int. .

Abstract

Recently, the use of herbs in the agriculture and food industry has increased significantly. In particular, Rosmarinus officinalis L. extracts have been reported to have strong antibacterial properties, which depend on their chemical composition. The present study displayed a biological method for synthesis of magnesium oxide (MgO) nano-flowers. The nano-flowers are developed without using any catalyst agent. Aqueous Rosemary extract was used to synthesize MgO nano-flowers (MgONFs) in stirring conditions and temperature at 70°C for 4 h. The mixture solution was checked by UV-Vis spectrum to confirm the presence of nanoparticles. The MgO nano-flowers powder was further characterized in this study by the X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. In addition, bacteriological tests indicated that MgO nano-flowers significantly inhibited bacterial growth, biofilm formation, and motility of Xanthomonas oryzae pv. oryzae, which is the causal agent of bacterial blight disease in rice. The electronic microscopic observation showed that bacterial cell death may be mainly due to destroy of cell integrity, resulting in leakage of intracellular content. As recommended, the use of Rosemary extract is an effective and green way to produce the MgO nano-flowers, which can be widely used in agricultural fields to suppress bacterial infection.

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Figures

Figure 1
Figure 1
Schematic of green synthesis of MgONFs using Rosmarinus officinalis L. (Rosemary).
Figure 2
Figure 2
UV-Vis spectrum of MgONFs.
Figure 3
Figure 3
XRD patterns of MgONFs.
Figure 4
Figure 4
FTIR spectrum of MgONFs.
Figure 5
Figure 5
TEM (a), SEM (b and c), observation and EDS spectrum (d) of MgONFs.
Figure 6
Figure 6
Antibacterial activity of MgO (a) and MgONFs (b) against Xoo strain GZ 0005.
Figure 7
Figure 7
Effects of MgONFs on growth (a), biofilm formation (b), and motility (c) of Xoo strain GZ 0005.
Figure 8
Figure 8
TEM graphs of Xoo strain GZ 0005 cells (a) and treated with 4 μg/ml MgONFs (b–d).
See this image and copyright information in PMC

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