Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Nov;75(11):3084-3092.
doi: 10.1002/ps.5425. Epub 2019 Apr 29.

Glutathione transferase plays a major role in flufenacet resistance of ryegrass (Lolium spp.) field populations

Rebecka Dücker  1   2 , Peter Zöllner  2 , Peter Lümmen  2 , Susanne Ries  2 , Alberto Collavo  2 , Roland Beffa  2
Affiliations

Glutathione transferase plays a major role in flufenacet resistance of ryegrass (Lolium spp.) field populations

Rebecka Dücker et al. Pest Manag Sci. 2019 Nov.

Abstract

Background: Herbicides inhibiting the synthesis of very long-chain fatty acids (HRAC group K3 , WSSA group 15), such as flufenacet, play an important role in weed management strategies, particularly when herbicide resistance to inhibitors with other modes of action, such as acetolactate synthase or acetyl coenzyme A carboxylase (ACCase), has already evolved. So far, only a few cases of resistance towards inhibitors of the synthesis of very long-chain fatty acids have been described. In this study, we characterized the level of flufenacet resistance in several Lolium spp. field populations and investigated the resistance mechanism.

Results: The screening for flufenacet resistance revealed the ability of Lolium spp. populations from several continents to survive flufenacet treatments at and above the field rate. This study demonstrates the way in which flufenacet is detoxified in resistant weed populations. Glutathione was found to be conjugated to flufenacet in Lolium spp. seedlings, and there was evidence that glutathione transferase activity was enhanced in protein extracts from flufenacet-resistant seedlings. A significant correlation was found between the resistance factor obtained by biotests and the degradation half-time of flufenacet in ryegrass plants obtained by high-performance liquid chromatography (HPLC).

Conclusion: At present, flufenacet resistance is not widespread; however, in certain Lolium spp. populations resistance levels could reach agronomic relevance due to detoxification by glutathione transferases. In Europe especially, only a few herbicide modes of action are registered for the control of Lolium spp. and therefore it is becoming increasingly important to apply best management practices to prevent the spread of flufenacet resistance. © 2019 Society of Chemical Industry.

Keywords: enhanced metabolism; flufenacet; glutathione transferases; non-target-site herbicide resistance; ryegrass; weed resistance.

PubMed Disclaimer

References

REFERENCES

    1. Burnet MWM, Hart Q, Holtum JAM and Powles SB, Resistance to nine herbicide classes in a population of rigid ryegrass (Lolium rigidum). Weed Sci 42:369-377 (1994).
    1. Powles SB and Yu Q, Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317-347 (2010).
    1. Yuan JS, Tranel PJ and Stewart CN, Non-target-site herbicide resistance: a family business. Trends Plant Sci 12:6-13 (2007).
    1. Heap I, The International Survey of Herbicide Resistant Weeds. [Online]. Available: http://www.weedscience.org [15 April 2019].
    1. Preston C, Tardif FJ, Christopher JT and Powles SB, Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pestic Biochem Physiol 54:123-134 (1996).

LinkOut - more resources