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. 2019 May 30;53(5):1802053.
doi: 10.1183/13993003.02053-2018. Print 2019 May.

Recognition of CD8+ T-cell epitopes to identify adults with pulmonary tuberculosis

Christina Lancioni  1 , Gwendolyn M Swarbrick  1   2 , Byung Park  3 , Melissa Nyendak  4 , Mary Nsereko  5 , Harriet Mayanja-Kizza  5   6 , Megan D Null  1 , Meghan E Cansler  1 , Rowan B Duncan  1 , Joy Baseke  5 , Keith Chervenak  7 , LaShaunda Malone  7 , Emily G Heaphy  7 , W Henry Boom  7 , David M Lewinsohn  1   2   8 , Deborah A Lewinsohn  1   8
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Recognition of CD8+ T-cell epitopes to identify adults with pulmonary tuberculosis

Christina Lancioni et al. Eur Respir J. .
No abstract available

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Conflict of interest statement

Conflict of interest: C. Lancioni has nothing to disclose. Conflict of interest: G.M. Swarbrick is an employee of ViTi, Inc, a company that may have commercial interest in the results of this research. This potential conflict has been reviewed and managed by OHSU. Conflict of interest: B. Park has nothing to disclose. Conflict of interest: M. Nyendak reports other from ViTi, Inc., outside the submitted work; Required language from OHSU: OHSU and M. Nyendak have a financial interest in ViTi, a company that may have a commercial interest in the results of this research and technology. These potential individual and institutional conflicts of interest have been reviewed and managed by OHSU. Conflict of interest: M. Nsereko has nothing to disclose. Conflict of interest: H. Mayanja-Kizza has nothing to disclose. Conflict of interest: M.D. Null is an employee of ViTi, Inc, a company that may have commercial interest in the results of this research. This potential conflict has been reviewed and managed by OHSU. Conflict of interest: M.E. Cansler is an employee of ViTi, Inc, a company that may have commercial interest in the results of this research. This potential conflict has been reviewed and managed by OHSU. Conflict of interest: R.B. Duncan has nothing to disclose. Conflict of interest: J. Baseke has nothing to disclose. Conflict of interest: K. Chervenak has nothing to disclose. Conflict of interest: L. Malone has nothing to disclose. Conflict of interest: E.G. Heaphy has nothing to disclose. Conflict of interest: W.H. Boom reports grants from NIH/NIAID, during the conduct of the study. Conflict of interest: D.M. Lewinsohn reports grants from NIH (HHSN272200900053C), grants from NIH (NO1-AI-95383 and HHSN266200700022C/NO1-AI-70022), other from Papé Family Research Institute, during the conduct of the study; other from ViTi Inc., outside the submitted work; OHSU and D.M. Lewinsohn have a financial interest in ViTi, a company that may have a commercial interest in the results of this research and technology. These potential individual and institutional conflicts of interest have been reviewed and managed by OHSU. Conflict of interest: D.A. Lewinsohn reports grants from NIH (HHSN272200900053C), grants from NIH (NO1-AI-95383 and HHSN266200700022C/NO1-AI-70022), other from Papé Family Research Institute (OHSU institutional funds), during the conduct of the study; other from ViTi Inc., outside the submitted work; OHSU and D.A Lewinsohn have a financial interest in ViTi, a company that may have a commercial interest in the results of this research and technology. These potential individual and institutional conflicts of interest have been reviewed and managed by OHSU.

Figures

FIGURE 1
FIGURE 1
CD8+ T-cell cytokine and tetramer responses to Mycobacterium tuberculosis (Mtb)-epitope pool distinguishes latent tuberculosis infection (LTBI) from tuberculosis (TB). a) The proportion of subjects with detectable CD8+ T-cell responses to a pool of eight Mtb-specific epitopes, as measured by interferon (IFN)-γ production in an intracellular cytokine staining (ICS) assay is shown (error bars indicate SEM). A positive response is defined as having at least 0.05% of CD8+ T-cells expressing IFN-γ after subtraction of background (no stimulation) responses. The proportion of positive responses between groups were compared using Chi-squared tests. b) The frequency of Mtb-specific CD8+ T-cells based on their production of either IFN-γ, tumour necrosis factor (TNF)-α and/or interleukin (IL)-2 is shown. The combinations of different cytokine functions are shown on the x-axis, with the frequency of distinct cytokine-producing cells within the Mtb-epitope pool responsive CD8+ T-cell compartment on the y-axis. The frequency of Mtb-specific CD8+ T-cells productive of each cytokine were compared using Kruskal–Wallis test, following adjustment for multiple comparisons using the Dwass, Steel, and Critchlow-Fligner method. c) The proportion of subjects with detectable CD8+ T-cell binding to a pool of eight Mtb-specific tetramers, as measured by flow cytometry, is shown (error bars indicate SEM). A positive response is defined as having at least 0.15% of CD8+ T-cells staining positive for tetramer. Statistical analysis with Chi-squared followed by pairwise comparison (Bonferroni adjustment). d) The frequencies of CD8+tetramer+ T-cells in different cohorts are shown. Statistical analysis with Kruskal–Wallis test followed by pairwise comparison (Bonferroni adjustment). Statistical Analysis System v9.4 (SAS Institute Inc., Cary, NC, USA) was used for analysis. #: p=0.02; : p=0.0009; +: p=0.012; §: p=0.006.
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References

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