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. 2019 Mar 28;8(13):e01594-18.
doi: 10.1128/MRA.01594-18.

Complete Genome Sequence of Cluster O Mycobacterium smegmatis Bacteriophage Ryadel

Travis Miller  1 , Danielle Bachhofer  1 , Ashleigh Cooper  1 , Jessica Doty  1 , Josh Katuri  1 , Jonathan Musgrave  1 , Aleksey Palumbo  1 , Heidi Spann  1 , Amanda Stone  1 , Keith Emmert  2 , Julie Edwards  1 , Jesse Meik  1 , James Pierce  1 , Dustin Edwards  3
Affiliations

Complete Genome Sequence of Cluster O Mycobacterium smegmatis Bacteriophage Ryadel

Travis Miller et al. Microbiol Resour Announc. .

Abstract

Mycobacteriophage Ryadel is a newly isolated cluster O Siphoviridae bacteriophage, characterized by an unusual prolate capsid, containing a 72,658-base-pair double-stranded DNA genome with 132 predicted protein-coding genes. Conserved among cluster O bacteriophages, the Ryadel genome contains 31 copies of a unique 17-bp sequence with dyad symmetry.

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Figures

FIG 1
FIG 1
Transmission electron microscopy (TEM) of mycobacteriophage Ryadel. Purified high-titer lysate was placed on a carbon type-B 300 mesh grid, stained with uranyl acetate, and imaged by a FEI Tecnai G2 Spirit BioTWIN (NL1.160G). TEM micrographs of negatively stained mycobacteriophage Ryadel showed a prolate capsid that was 160 nm in length by 40 nm in width (4:1 ratio), a morphology that corresponds to that of members of cluster O bacteriophages within the Siphoviridae family.
See this image and copyright information in PMC

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