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. 1986 Aug;52(2):394-5.
doi: 10.1128/aem.52.2.394-395.1986.

High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA

D Simon, A Rouault, M C Chopin

High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA

D Simon et al. Appl Environ Microbiol. 1986 Aug.

Abstract

Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).

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References

    1. Appl Environ Microbiol. 1984 Aug;48(2):347-51 - PubMed
    1. Appl Microbiol. 1975 Jun;29(6):807-13 - PubMed
    1. Appl Environ Microbiol. 1982 May;43(5):1213-5 - PubMed
    1. Plasmid. 1984 May;11(3):260-3 - PubMed
    1. J Bacteriol. 1982 Mar;149(3):824-30 - PubMed

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