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Review
. 2019 Jun:50:37-44.
doi: 10.1016/j.cbpa.2019.02.005. Epub 2019 Mar 28.

Developing zebrafish disease models for in vivo small molecule screens

Pui-Ying Lam  1 , Randall T Peterson  2
Affiliations
Review

Developing zebrafish disease models for in vivo small molecule screens

Pui-Ying Lam et al. Curr Opin Chem Biol. 2019 Jun.

Abstract

The zebrafish is a model organism that allows in vivo studies to be performed at a scale usually restricted to in vitro studies. As such, the zebrafish is well suited to in vivo screens, in which thousands of small molecules are tested for their ability to modify disease phenotypes in zebrafish disease models. Numerous approaches have been developed for modeling human diseases in zebrafish, including mutagenesis, transgenesis, pharmacological approaches, wounding, and exposure to infectious or cancerous agents. We review the various strategies for modeling human diseases in zebrafish and discuss important considerations when developing zebrafish models for use in in vivo small molecule screens.

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Figures

Fig. 1.
Fig. 1.
Schematic representation of the six different general approaches for generating zebrafish disease model for chemical screening.
See this image and copyright information in PMC

References

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