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. 2019 Sep;139(9):2061-2064.e2.
doi: 10.1016/j.jid.2019.02.032. Epub 2019 Mar 29.

CCR6+ γδ T Cells Home to Skin Wounds and Restore Normal Wound Healing in CCR6-Deficient Mice

Leif S Anderson  1 , Sebastian Yu  2 , Kathryn R Rivara  1 , Mack B Reynolds  1 , Alfredo A Hernandez  1 , Xuesong Wu  2 , Hsin-Ya Yang  2 , Roslyn R Isseroff  2 , Lloyd S Miller  3 , Sam T Hwang  2 , Scott I Simon  4
Affiliations

CCR6+ γδ T Cells Home to Skin Wounds and Restore Normal Wound Healing in CCR6-Deficient Mice

Leif S Anderson et al. J Invest Dermatol. 2019 Sep.
No abstract available

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Figures

Figure 1:
Figure 1:. CCR6−/− mice have hindered dermal wound healing, defective γδ T cell trafficking to a wound, and dysregulated IL-17A and FGF2 expression in the wound.
WT and CCR6−/− mice were administered a 6mm full thickness skin wound on the dorsum. (a-c) Wound size was measured daily. γδ T cells and Vγ4 T cells were enumerated from (e) wounds, (f) lymph nodes, and (g) blood by flow cytometry (d). Unwounded skin (day 0) or day 5 wounds were collected from animals and expression of cytokines was determined by RT-qPCR (h). Animals per group are presented in each graph and 4–5 animals per group for (h). Data is presented as Mean ± SEM. *P ≤ 0.05 and **P ≤ 0.01 between CCR6−/− and WT. Scale bar = 5 mm.
Figure 2:
Figure 2:. Adoptive transfer of γδ T cells restores wound healing in CCR6−/− mice.
(a) γδ T cells were enriched from the lymph nodes of WT and CCR6−/− mice and stained with the membrane dye VT680. 100,000 γδ T cells were transferred into mice via the tail vein. Mice were wounded the subsequent day, and (b) wound size was measured daily. (c and d) VT680 signal was measured for 5 days to measure γδ T cell trafficking. Data is presented as Mean ± SEM. * P **P ≤ 0.01 and ***P ≤ 0.001 comparing CCR6−/− mice receiving WT γδ T cells to other groups using a repeated measures two-way analysis of variance followed by Bonferroni’s post hoc test (b and c) Scale bar = 3 mm.
See this image and copyright information in PMC

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