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. 2019 Mar;14(1):12-16.
doi: 10.1016/j.joto.2018.11.002. Epub 2018 Nov 10.

Chronic model of tympanic perforation in rats with mitomycin C and dexamethasone

Tomás Esteban  1 , Noemí M Atucha  2 , José María Moraleda  3 , Joaquín García-Estañ  2 , Carmen L Insausti  3 , Javier Moraleda-Deleyto  4
Affiliations

Chronic model of tympanic perforation in rats with mitomycin C and dexamethasone

Tomás Esteban et al. J Otol. 2019 Mar.

Erratum in

Abstract

A rat model of chronic tympanic membrane perforation was developed to be used in the search of new materials for the sealing of these perforations. A longitudinal study was carried out in rats subjected to incisional myringotomy followed by the application of mitomycin C alone or with dexamethasone. Rats were checked at days 3, 7, 10, 14 and weekly thereafter until perforation closure, for up to 6 months. The addition of dexamethasone is a key component in order to obtain a chronic opening. Myringotomies treated with saline had a mean healing time of 8.5 days. At 8 weeks, between 62.5% and 77.7% of tympanic membranes treated with mitomycin C and dexamethasone remained perforated and at 6 months this number fell to 21.4%. This technique is able to maintain most tympanic membrane perforations patent for at least 8 weeks. This rat model is adequate for its use in preclinical or translational research.

Keywords: Animal model; Chronic tympanic membrane perforation; Dexamethasone; Mitomycin C; Myringotomy.

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Figures

Fig. 1
Fig. 1
Camera with optical adapter (right) and tympanoplasty instruments used (left).
Fig. 2
Fig. 2
Perforated eardrums of groups A, B and C at 8 weeks.
Fig. 3
Fig. 3
Healing time of tympanic membranes treated with mitomycin C.
Fig. 4
Fig. 4
Perforated eardrums at 6 months (one of subgroup C1 (left image) and two of C2).
Fig. 5
Fig. 5
Healing time of tympanic membranes treated with saline solution.
See this image and copyright information in PMC

References

    1. Babu S.C., Kartush J.M., Patni A. Otologic effects of topical mitomycin C: phase I-evaluation of ototoxicity. Otol. Neurotol. 2005;26(2):140–144. - PubMed
    1. Baumans V. The welfare of laboratory mice. In: En: Laliste E., editor. The Welfare of Laboratory Animals. Springer; Dordrecht: 2007. pp. 119–152.
    1. BOE Ley 32/2007 para el cuidado de los animales en su explotación, transporte, experimentación y sacrificio. BOE. 2007;268(8 Noviembre 2007):45914–45920. https://www.boe.es/buscar/doc.php?id=BOE-A-2007-19321 2007.
    1. Directiva 2010/63/UE del Parlamento Europeo y del Consejo de 22 de Septiembre, relativa a la protección de los animales utilizados para fines científicos, (http://eur-lex.europa.eu/legal-content/ES/TXT/?uri=LEGISSUM%3Asa0027).
    1. Insausti C.L., Blanquer M., Majado M.J., Insausti A., Moraleda J.M. Utilidad terapéutica potential de las células madre de la membrana amniótica. Revista de Hematología mexicana. 2011;11(4):276–286.