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. 2019 Aug 27;400(9):1141-1146.
doi: 10.1515/hsz-2019-0111.

Sub-membranous actin rings in the axon initial segment are resistant to the action of latrunculin

Amr Abouelezz  1   2 , David Micinski  1 , Aino Lipponen  1 , Pirta Hotulainen  1
Affiliations

Sub-membranous actin rings in the axon initial segment are resistant to the action of latrunculin

Amr Abouelezz et al. Biol Chem. .

Abstract

The axon initial segment (AIS) comprises a sub-membranous lattice containing periodic actin rings. The overall AIS structure is insensitive to actin-disrupting drugs, but the effects of actin-disrupting drugs on actin rings lack consensus. We examined the effect of latrunculin A and B on the actin cytoskeleton of neurons in culture and actin rings in the AIS. Both latrunculin A and B markedly reduced the overall amount of F-actin in treated neurons in a dose-dependent manner, but the periodicity of actin rings remained unaffected. The insensitivity of AIS actin rings to latrunculin suggests they are relatively stable.

Keywords: cell biology; neurobiology; neuron.

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