Liquid assisted pulsed laser ablation synthesized copper oxide nanoparticles (CuO-NPs) and their differential impact on rice seedlings

Abstract

Hydroponic experiments were conducted to investigate impact of laser ablated copper oxide nanoparticles (CuO-NPs) on rice seedlings. The present work demonstrates that exposure of lower concentrations (5, 10, 20, and 50 µM) of CuO-NPs enhance growth (in terms of fresh and dry weight and length), of rice seedlings. However, at higher concentrations (100, 200, and 500 µM) of CuO-NPs, growth (in terms of length, fresh weight and dry weight) decreased significantly (P < 0.05). Further, photosynthetic pigments (total chlorophyll and carotenoids) and protein contents were also found to be in accordance with the results of growth. This had occurred due to enhanced level of CuO-NPs accumulation at higher doses which also enhanced the level of oxidative stress markers such as hydrogen peroxide (H₂O₂) and malondialdehyde (MDA). Chlorophyll a fluorescence parameters (F_v/F_m and qP and except NPQ) and amount of some minerals (Ca, Mg, Na, and K) increased at lower concentrations of CuO-NPs. In contrast, the levels of F_v/F_m and qP were significantly (P < 0.05) reduced at higher concentration of CuO-NPs, which might be due to enhanced accumulation of Cu and oxidative stresses markers. Our results showed that lower dosages of pulsed laser ablated CuO-NPs (5, 10, 20, and 50 µM) might be beneficial for growth and development of rice seedlings.

Keywords: Chlorophyll fluorescence; CuO-NPs; Growth parameters; Liquid assisted pulsed laser ablation; Rice seedlings.