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. 1986 Sep;18(3):421-4.
doi: 10.1093/jac/18.3.421.

An ultra-rapid method for the study of antibiotic resistance plasmids

P M Bennett, J Heritage, P M Hawkey

An ultra-rapid method for the study of antibiotic resistance plasmids

P M Bennett et al. J Antimicrob Chemother. 1986 Sep.

Abstract

A rapid method of isolating plasmid DNA has been developed from that of Birnboim & Doly (1979). This method allows large numbers of strains to be examined, and can be employed to isolate DNA from members of the Enterobacteriaceae, Pseudomonas aeruginosa, Staphylococcus aureus, (including methicillin-resistant strains) and coagulase-negative staphylococci. Plasmids of widely differing sizes are amenable to isolation by this technique, which yields DNA of sufficient purity to allow restriction endonuclease and homoduplex analysis.

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