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. 2019 Jan 2:18:1-7.
eCollection 2019.

Seromolecular assess of Toxoplasma gondii infection in pregnant women and neonatal umbilical cord blood

Mohammad Menati Rashno  1 , Shirzad Fallahi  2 , Zahra Arab-Mazar  3 , Hassan Dana  1
Affiliations

Seromolecular assess of Toxoplasma gondii infection in pregnant women and neonatal umbilical cord blood

Mohammad Menati Rashno et al. EXCLI J. .

Abstract

Toxoplasmosis is considered as one of the most prevalent human parasitic infections that can be transmitted from mother to the fetus. The onset of toxoplasmosis during pregnancy has clinical complications including spontaneous abortion, preterm labor, stillbirth and fetal abnormalities. The aim of this study was to investigate the prevalence of Toxoplasmosis infection in pregnant women and their infants in Lorestan province, Western Iran. Blood and sera samples were collected from 98 pregnant women and their infants. All collected samples were examined for Toxoplasma gondii infection by serological tests (ELISA IgM & IgG) and PCR assay. Among the 98 samples of mother and umbilical cord prevalence of anti-Toxoplasma IgG, was 34/98 (34.69 %) and 33/98 (33.67 %), respectively. All pregnant women were negative for, anti-Toxoplasma IgM while it was found in 5/98 (5.1 %) of umbilical cords. Based on PCR analysis, Toxoplasma infection was detected in 5 (5.1 %) and 7 (7.14 %) of mother and umbilical cords, respectively. Molecular test along with evaluation of IgM (P <0.001) and IgG (P = 0.001) were significantly correlated.

Keywords: cord blood; molecular methods; pregnant women; serological methods.

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Figures

Table 1
Table 1. Results from methods performed on maternal and infants blood samples
Table 2
Table 2. Comparison and correlation between the history of abortion and molecular and serological methods
Table 3
Table 3. Comparison and correlation of both serological and molecular methods
Figure 1
Figure 1. The studied area. Iran map, Lorestan province and Khorramabad city are marked in red
Figure 2
Figure 2. PCR product electrophoresis mothers and babies with toxoplasmosis in Khorramabad city
See this image and copyright information in PMC

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