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. 2018 Oct 23;28(2):423-432.
doi: 10.1007/s10068-018-0493-z. eCollection 2019 Apr.

New formulation of vitamin C encapsulation by nanoliposomes: production and evaluation of particle size, stability and control release

Samaneh Amiri  1 , Mahmoud Rezazadeh-Bari  1 , Mohammad Alizadeh-Khaledabad  1 , Saber Amiri  2
Affiliations

New formulation of vitamin C encapsulation by nanoliposomes: production and evaluation of particle size, stability and control release

Samaneh Amiri et al. Food Sci Biotechnol. .

Abstract

In the present study, the effects of different ratios of milk phospholipids, cholesterol and phytosterols (Campesterol) powder (50-100%, 0-50%, and 0-50%, respectively) and sonication time (20, 25, 30, 35 and 40 min) were investigated to produce a new formulation of nanoliposomes for encapsulation of vitamin C. The results showed that increasing the time of sonication and decreasing the ratio of phospholipid to phytosterol significantly decreased nanoliposomes' particle size (p < 0.05). The maximum encapsulation efficiency was obtained at 35 and 40 min of sonication time and 75-25 ratio of phospholipid: phytosterol. Also, reducing the sonication time in the same ratio of phospholipid/phytosterol caused to increase the controlled release. The highest stability of vitamin C during 20 days was obtained in the ratio of 75-25 (phospholipids: campesterol). The results showed a positive effect of cholesterol replacement with campesterol on encapsulation efficiency, control release and stability of vitamin C in nanoliposomes.

Keywords: Campesterol; Milk phospholipids; Nanoliposomes; Ultrasonic waves.

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Figures

Fig. 1
Fig. 1
(A) The effect of phospholipid to cholesterol ratio and time of sonication on encapsulation efficiency, (B) Changes in encapsulation efficiency at the time of sonication and different ratios of phospholipid/phytosterol
Fig. 2
Fig. 2
(A) The effect of three factors, phospholipid: cholesterol: phytosterol in the average of produced particle size, (B) The effect of the ratio of phospholipid: phytosterol and the time of sonication on the standard deviation of particle size
Fig. 3
Fig. 3
(A) Vitamin C stability during sonication and different ratios of cholesterol to phytosterol, (B) Stability changes during sonication and different ratios of phospholipid to phytosterol, (C) Effect of sonication time and cholesterol/phytosterol ratio on vitamin C stability after 10 days, (D) Time of Sonication and the ratio of phospholipid to phytosterol in vitamin C stability after 10 days, (E) The effect of three factors phospholipid: cholesterol: phytosterol on the stability of vitamin C after 10 days
Fig. 4
Fig. 4
(A) The effects of the phospholipid to phytosterol ratio and the time of sonication on vitamin C stability after 20 days of storage, (B) Impact of sonication time and the ratio of phospholipid to cholesterol on the stability of vitamin C after 20 days, (C) The effects of changes in cholesterol/phytosterol ratio and sonication time on vitamin C stability after 20 days
Fig. 5
Fig. 5
(A) The ratio of phospholipid to cholesterol, and the effect of time of sonication on control release, (B) The effects of cholesterol to phytosterol ratio and sonication time on RR%, (C) The effects of three phospholipids, cholesterol and phytosterol factors on control of vitamin C release in samples
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