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. 1986 Oct;52(4):689-95.
doi: 10.1128/aem.52.4.689-695.1986.

Method for flow cytometric detection of Listeria monocytogenes in milk

C W Donnelly, G J Baigent

Method for flow cytometric detection of Listeria monocytogenes in milk

C W Donnelly et al. Appl Environ Microbiol. 1986 Oct.

Abstract

This report describes a method for the detection of Listeria monocytogenes in raw milk by flow cytometric analysis of fluorescently labeled bacterial populations. The use of immunofluorescence in combination with measures of DNA content by propidium iodide labeling and size by light scattering enabled specific identification of L. monocytogenes from Streptococcus faecalis, Streptococcus agalactiae, Streptococcus uberis, Staphylococcus epidermidis, and Staphylococcus hyicus. Additional specific resolution of L. monocytogenes populations was achieved through selective enrichment of raw milk in Listeria enrichment broth. These procedures should permit the rapid screening of milk and other food samples for L. monocytogenes and eliminate many of the short-comings associated with conventional fluorescent-antibody procedures.

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References

    1. J Clin Microbiol. 1985 Oct;22(4):677-9 - PubMed
    1. J Dent Res. 1984 Aug;63(8):1040-2 - PubMed
    1. N Engl J Med. 1983 Jan 27;308(4):203-6 - PubMed
    1. Bacteriol Rev. 1966 Jun;30(2):309-82 - PubMed
    1. Zentralbl Bakteriol Orig A. 1977 Sep;239(1):62-9 - PubMed

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