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. 2019 Apr 26;39(4):BSR20182057.
doi: 10.1042/BSR20182057. Print 2019 Apr 30.

Clinical significance of high expression of stanniocalcin-2 in hepatocellular carcinoma

Yuan Wang  1   2 , Jian Wu  3 , Jiangyan Xu  4 , Shengyou Lin  5
Affiliations

Clinical significance of high expression of stanniocalcin-2 in hepatocellular carcinoma

Yuan Wang et al. Biosci Rep. .

Abstract

To investigate the significance of stanniocalcin-2 (STC2) expression in hepatocellular carcinoma (HCC) tissues and adjacent tissues. Levels of STC2 in HCC tissue were detected in 200 HCC patients tissues and adjacent tissues as controls by immunohistochemistry technique (IHC) and reverse transcriptase-PCR (RT-PCR). Single factor analysis was used to study the relationship between expression of STC2 mRNA and protein and clinicopathological features of HCC. Multifactor Cox survival analysis was used to relationship between the expression of STC2 and overall survival of postoperative patients with HCC. IHC staining showed that the expression of STC2 protein rate was 81.00% (163/200). And the positive rate of adjacent tissues was 29.00% (58/200). Western blot showed that the expression of STC2 protein in HCC was significantly higher than that in the adjacent tissues (P<0.05). RT-PCR showed that the positive rates of STC2 mRNA expression in HCC were 75.50% (151/200), which was significantly higher than that in adjacent tissues 14.50% (29/200) (P<0.05). Both STC2 mRNA and protein expression are related to tumor diameter, stage, tumor metastasis, carcinoma emboli in the portal vein and the degree of tumor differentiation in HCC. The HCC patients with higher expression of STC2 had shorter median survival time. STC2 expression, tumor diameter, carcinoma emboli in the portal vein, tumor differentiation degree, and tumor stage were independent factors affecting the overall survival of postoperative patients. The high expression of STC2 mRNA and protein expression in HCC may be associated with the occurrence, development, and prognosis of HCC. STC2 may also be possible to help developing new therapeutic strategies for HCC.

Keywords: Stanniocalcin-2; hepatocellular carcinoma; prognosis; therapeutic strategies.

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Conflict of interest statement

The authors declare that there are no competing interests associated with the manuscript.

Figures

Figure 1
Figure 1. The staining result of EnVision immunohistochemistry for STC2 in HCC and adjacent tissues (×200)
(A) The staining weakly positive result for STC2 in adjacent tissues (×100); (B) the staining weakly positive result for STC2 in adjacent tissues (×400); (C) the staining strongly positive result for STC2 in HCC tissues (×100); (D) the staining strongly positive result for STC2 in HCC tissues (×400). **P<0.05 .
Figure 2
Figure 2. The expression of STC2 protein and STC2 mRNA in HCC and adjacent tissues
(A) expression of STC2 protein in HCC and adjacent tissues; (B) expression of STC2 mRNA in HCC and adjacent tissues; (A) negative control group; (B) adjacent tissues; (C) HCC tissues. **P<0.05.
Figure 3
Figure 3. STC2 expression and survival analysis of HCC patients
Figure 4
Figure 4. Association of STC2 expression and prognosis of HCC patients: multifactor Cox survival
See this image and copyright information in PMC

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