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. 2018 May 3;9(2):85-89.
doi: 10.1016/j.jtcme.2018.02.003. eCollection 2019 Apr.

Phytochemical evaluation and anti-hemorrhoidal activity of bark of Acacia ferruginea DC

Samriti Faujdar  1 , Bhawana Sati  1 , Swapnil Sharma  1 , A K Pathak  2 , Sarvesh Kumar Paliwal  1
Affiliations

Phytochemical evaluation and anti-hemorrhoidal activity of bark of Acacia ferruginea DC

Samriti Faujdar et al. J Tradit Complement Med. .

Erratum in

  • Erratum for previously published articles.
    [No authors listed] [No authors listed] J Tradit Complement Med. 2020 Sep 28;10(6):605. doi: 10.1016/j.jtcme.2020.09.001. eCollection 2020 Nov. J Tradit Complement Med. 2020. PMID: 33134138 Free PMC article.

Abstract

The present study has been carried out to evaluate antihemorrhoidal activity of bark of Acacia ferruginea DC. The total phenolic, total flavonoid and saponins were determined. Anti-hemorrhoidal potential of bark extract was determined by levels of inflammatory cytokines such as TNF-α, IL-6, PGE2 and recto-anal coefficient (RAC). The histopathological examination was done to evaluate the severity score in the treated and untreated groups. The results of phytochemical screening of the hydroalcoholic extract of A. ferruginea revealed the presence of alkaloids, flavonoids, triterpenoid, saponins, tannins and phenolic compounds The total phenolic, flavonoid and saponin contents were found to be 438.8mg/g GAE, 66.6mg/gRE and 34%w/w respectively. Hydroalcoholic extract of bark of A. ferruginea significantly reduced the inflammatory cytokines {TNF-α(8.40±0.188), IL-6(3.95±0.181), PGE2(53.27±2.956) and RAC(0.998±0.094)}as compared to positive control group{TNF-α(13.36±0.141), IL-6(7.25±0.161), PGE2(82.34±3.395) and RAC(1.131 ± 0.008)}. Noticeably the results were comparable to that of standard pilex granules {TNF-α (7.12±0.166), IL-6(3.01±0.156), PGE2(42.51±2.157) and RAC (0.968±0.084)}. Molecular docking and structure based pharmacophore mapping further confirmed the anti-inflammatory mediated antihemorrhoidal activity of the hydroalcoholic extract. The antihemorrhoidal activity of hydroalcoholic extract of A. ferruginea may attribute to the flavonoids.

Keywords: Acacia ferruginea; Anti-hemorrhoidal; Molecular docking; Total phenolic content.

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Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Effect of Bark extract and Pilex granules on the histology of rectoanal tissue in croton-oil induced hemorrhoids. Rectoanal sections of rats: Fig. 1 (a) Group I (normal control) has normal architecture of rectal tissue; (b) Group II (positive control) exhibited severe inflammation, hemorrhage, necrosis, vasodilation and congestion (c) Group III treated with hydroalcoholic extract of bark showed minimal inflammation, congestion, hemorrhage, dilatation, degeneration and necrosis (d) Group IV treated with pilex granules (400 mg/kg p.o.) showed nearly normal architecture of the tissue.
Fig. 2
Fig. 2
Pharmacophore mapping of a) oxaprozin b) 5-(3,4-dimethylphenyl)-4-phenylisoxazole.
Fig. 3
Fig. 3
Docking pose of a) Oxaprozin b) 5-(3,4-dimethylphenyl)-4-phenylisoxazole.
See this image and copyright information in PMC

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