Biocompatible biodegradable polycaprolactone/basil seed mucilage scaffold for cell culture

Abstract

In this study, a polymer obtained from the basil seed mucilage (BSM) in combination with polycaprolactone (PCL), was used in the 2D scaffold production process for cell culture. First, combinations of two polymers with different ratios and concentrations were prepared and electrospun. Among these samples, a sample with a BSM/PCL ratio of 2/3 was used to perform different tests due to its fibre uniformity and appropriate diameter. The Fourier transform infrared spectrometer test was carried out to chemically analyse the scaffold, the X-ray diffraction test to determine the crystallinity of the scaffold, and the contact angle test to determine the hydrophilicity of the scaffold. The strength, porosity, and degradation percentage of the scaffold were also studied. With appropriate conditions of the scaffold for cell culture determined, Vero epithelial cells were cultured on the scaffold. Results obtained from cell culture indicated that the adhesion of the scaffold was suitable for the appropriate growth cells.

Fig. 1

Morphology of (A)–(C) PCL/BSM 75/25 and (D)–(F) 60/40

Fig. 2

XRD pattern of PCL, BSM and PCL/BSM fibre (75/25)

Fig. 3

FTIR pattern of PCL, BSM and fibre

Fig. 4

Percentage of in vivo degradation of nanofibrous scaffolds versus time after soaking in PBS

Fig. 5

Stress–strain curves of (A) PCL and (B) Fibre

Fig. 6

MTT results of vero on cell culture plate, PCL and PCL/BSM nanofibres after 24, 48 and 72 h of cell seeding. Different small letters indicate significant differences between different scaffold/samples on the same time period (p < 0.05); different capital letters represent significant differences between the same sample on a different time (P < 0.05)

Fig. 7

Cell morphology on (a) , (b) PCL scaffold after 24 and 72 h of cell cultured. (c), (d) PCL/BSM scaffold after 24 and 72 h of cell cultured