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. 2019 Feb;13(1):66-70.
doi: 10.1049/iet-nbt.2018.5212.

Investigation of ZnO nanoparticles on proline, anthocyanin contents and photosynthetic pigments and lipid peroxidation in the soybean

Shahla Hashemi  1 , Zahra Asrar  2 , Shahram Pourseyedi  3 , Nazi Nadernejad  2
Affiliations

Investigation of ZnO nanoparticles on proline, anthocyanin contents and photosynthetic pigments and lipid peroxidation in the soybean

Shahla Hashemi et al. IET Nanobiotechnol. 2019 Feb.

Abstract

The interaction between nanoparticles and plants is inevitable. In this study, the effect of different concentrations of ZnO nanoparticles synthesised using olive extract on the soybean was studied. The soybean seeds were cultured in a Hoagland medium containing agar which was treated different concentrations (0, 200 and 400 ppm) of ZnO nanoparticles. After 21 days, the plants were harvested and the parameters of proline, anthocyanin, malondialdehyde (MDA), hydrogen peroxide (H2O2), chlorophyll and carotenoid contents and phenylalanine ammonia-lyase (PAL) and catalase (CAT) activity in soybeans (Glycine max) were measured. The results showed that the levels of chlorophyll a and b and carotenoid at concentrations of 200 and 400 ppm in comparison with control decreased, while carotenoid content at 200 ppm concentration at a concentration of 400 ppm was not significant. The level of anthocyanin and PAL activity increased with increasing concentration of nanoparticles, while proline content decreased. By increasing the concentration of ZnO nanoparticles, the content of MDA and hydrogen peroxide increased compared to control but CAT activity did not change significantly. This research suggests that ZnO nanoparticles synthesised using olive extract in soybean plants may be toxic by reactive oxygen species production.

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Figures

Fig. 1
Fig. 1
UV–visible spectroscopy of ZnO nanoparticles [41]
Fig. 2
Fig. 2
TEM image of ZnO nanoparticles [41]
Fig. 3
Fig. 3
Effect of treatment of zinc oxide nanoparticles on chlorophyll content in soybeans. Vertical bars indicate ± standard error
Fig. 4
Fig. 4
Effect of treatment of zinc oxide nanoparticles on carotenoid content in soybeans. Vertical bars indicate ± standard error
Fig. 5
Fig. 5
Effect of treatment of zinc oxide nanoparticles on anthocyanin content in soybeans. Vertical bars indicate ± standard error
Fig. 6
Fig. 6
Effect of treatment of zinc oxide nanoparticles on PAL activity in soybeans. Vertical bars indicate ± standard error
Fig. 7
Fig. 7
Effect of treatment of zinc oxide nanoparticles on proline content in soybeans. Vertical bars indicate ± standard error
Fig. 8
Fig. 8
Effect of treatment of zinc oxide nanoparticles on MDA content in soybeans. Vertical bars indicate ± standard error
Fig. 9
Fig. 9
Effect of treatment of zinc oxide nanoparticles on H2 O2 content in soybeans. Vertical bars indicate ± standard error
Fig. 10
Fig. 10
Effect of treatment of zinc oxide nanoparticles on CAT activity in soybeans. Vertical bars indicate ± standard error
See this image and copyright information in PMC

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