Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2018 Dec;148(Suppl):S115-S119.
doi: 10.4103/ijmr.IJMR_2096_17.

Approaches used to improve epigenetic reprogramming in buffalo cloned embryos

Monika Saini  1 , Naresh L Selokar  1
Affiliations
Review

Approaches used to improve epigenetic reprogramming in buffalo cloned embryos

Monika Saini et al. Indian J Med Res. 2018 Dec.

Abstract

The reproductive cloning in buffalo in India has been started using a simplified somatic cell nuclear transfer technique named handmade cloning. Since the birth of first cloned female buffalo in 2009, a number of buffalo clones have been produced in India by utilizing different types of donor cells such as ear cells, embryonic stem cells, semen somatic cells and urine somatic cells. The use of buffalo cloning on a large scale is restricted due to low pregnancy rates and poor calf survival. Considerable attempts have been made to improve the overall buffalo cloning efficiency, particularly by modifying epigenetic reprogramming of cloned embryos. Previous studies have demonstrated that chemical epigenetic modifiers such as trichostatin A and 5-aza-2'-deoxycytidine, m-carboxycinnamic acid bishydroxamide can be used to treat donor somatic cells and reconstructed fused embryos to correct the epigenetic reprogramming to enhance the overall cloning efficiency in terms of live birth rates.

Keywords: Birth rate; buffalo; cloned; embryos; epigenetics; reprogramming.

PubMed Disclaimer

Conflict of interest statement

None

Figures

Figure
Figure
Different approaches used to correct an epigenetic reprogramming in buffalo cloned embryos. Somatic cell, oocyte, and fused embryo can be treated with any mentioned method either individually or in combination to improve reprogramming. BCB, Brilliant Cresyl Blue. Source: Ref. .
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH. Viable offspring derived from fetal and adult mammalian cells. Nature. 1997;385:810–3. - PubMed
    1. Keefer CL. Artificial cloning of domestic animals. Proc Natl Acad Sci U S A. 2015;112:8874–8. - PMC - PubMed
    1. Saini M, Selokar NL, Palta P, Chauhan MS, Manik RS, Singla SK, et al. An update: Reproductive handmade cloning of water buffalo (Bubalus bubalis) Anim Reprod Sci. 2018;197:1–9. - PubMed
    1. Selokar NL, Saini M, Palta P, Chauhan MS, Manik RS, Singla SK. Cloning of buffalo, a highly valued livestock species of South and Southeast Asia: Any achievements? Cell Reprogram. 2018;20:89–98. - PubMed
    1. Yang F, Hao R, Kessler B, Brem G, Wolf E, Zakhartchenko V, et al. Rabbit somatic cell cloning: Effects of donor cell type, histone acetylation status and chimeric embryo complementation. Reproduction. 2007;133:219–30. - PubMed