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Review
. 2018 Dec;148(Suppl):S115-S119.
doi: 10.4103/ijmr.IJMR_2096_17.

Approaches used to improve epigenetic reprogramming in buffalo cloned embryos

Monika Saini  1 , Naresh L Selokar  1
Affiliations
Review

Approaches used to improve epigenetic reprogramming in buffalo cloned embryos

Monika Saini et al. Indian J Med Res. 2018 Dec.

Abstract

The reproductive cloning in buffalo in India has been started using a simplified somatic cell nuclear transfer technique named handmade cloning. Since the birth of first cloned female buffalo in 2009, a number of buffalo clones have been produced in India by utilizing different types of donor cells such as ear cells, embryonic stem cells, semen somatic cells and urine somatic cells. The use of buffalo cloning on a large scale is restricted due to low pregnancy rates and poor calf survival. Considerable attempts have been made to improve the overall buffalo cloning efficiency, particularly by modifying epigenetic reprogramming of cloned embryos. Previous studies have demonstrated that chemical epigenetic modifiers such as trichostatin A and 5-aza-2'-deoxycytidine, m-carboxycinnamic acid bishydroxamide can be used to treat donor somatic cells and reconstructed fused embryos to correct the epigenetic reprogramming to enhance the overall cloning efficiency in terms of live birth rates.

Keywords: Birth rate; buffalo; cloned; embryos; epigenetics; reprogramming.

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Conflict of interest statement

None

Figures

Figure
Figure
Different approaches used to correct an epigenetic reprogramming in buffalo cloned embryos. Somatic cell, oocyte, and fused embryo can be treated with any mentioned method either individually or in combination to improve reprogramming. BCB, Brilliant Cresyl Blue. Source: Ref. .
See this image and copyright information in PMC

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