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. 2017 Jun 9;9(6):214.
doi: 10.3390/polym9060214.

Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for Paeoniflorin

Wei Zhang  1 , Bofeng Wei  2 , Shoujiang Li  3 , Yueming Wang  4 , Shaoyan Wang  5   6
Affiliations

Preparation and Chromatographic Application of β-Cyclodextrin Molecularly Imprinted Microspheres for Paeoniflorin

Wei Zhang et al. Polymers (Basel). .

Abstract

The application of molecular imprinting technology in the separation and purification of active ingredients in natural products was widely reported, but remains a challenge. Enrichment and separation are especially limited. A surface imprinting technique was reported to synthesize molecularly imprinted microspheres (MIMs) in this article. With paeoniflorin (PF) as the template molecule, β-cyclodextrin (β-CD) and acrylamide (AA) as the functional monomers, and poly(glycidyl methacrylate, GMA) microspheres (PGMA) as the backing material. MIMs have been characterized by FTIR and FESEM. Adsorption experiments indicated the adsorption capacity of MIMs was superior to those comparative non-imprinted microspheres (NIMs) and the binding isotherm of MIMs was in good agreement with the two-site binding model. The baseline separation of PF and its structural analogue albiflorin (AF) were achieved on the new MIMs packed column. MIMs showed good affinity and efficiency for separation of PF and AF compared with those comparative NIMs. The approach of fabricating MIMs is simple, rapid, and inexpensive, and may shed new light on the application of MIMs as a liquid chromatography stationary phase to separate and analyze PF and AF from the Red peony root extracts.

Keywords: chromatographic application; molecular imprinting; paeoniflorin; stationary phase.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Structure of β-cyclodextrin (β-CD), paeoniflorin (PF), and albiflorin (AF): (a) β-CD; (b) PF; (c) AF.
Figure 2
Figure 2
Scheme for preparation of poly(glycidyl methacrylate) microspheres (PGMA)-cyclodextrin (CD) and molecularly imprinted microspheres (MIMs).
Figure 3
Figure 3
FTIR spectra of PGMA (a), β-CD (b), and MIMs-1 (c).
Figure 4
Figure 4
Scanning electron micrographs of PGMA (a) and MIMs-1 (b).
Figure 5
Figure 5
The adsorption dynamics curves of the MIMs and NIMs. Adsorption conditions: 10 mL of 10 μmol·mL−1 solutions (30% methanol) of PF with 50 mg of MIMs or NIMs.
Figure 6
Figure 6
The Scatchard plots of MIMs-1 (a) and NIMs-1 (b) for PF.
Figure 7
Figure 7
The fitting curve of equilibrium binding equation.
Figure 8
Figure 8
Selectivity study of PF and AF with MIMs and NIMs.
Figure 9
Figure 9
Chromatograms of PF and AF on MIMs-1/NIMs-1 (a) and Chromatogram of Red peony root extracts on MIMs-1 (b). LC conditions of MIMs-1/NIMs-1 (a,b): mobile phase: methanol–water–acetic acid (27:73:0.06, v/v/v); flow rate was 0.5 mL·min−1; extracting conditions of Red peony root extracts: 10 g of Red peony root powder was dissolved in 40 mL of 50% ethanol solution (v %), the extraction temperature was 50 °C, and the time was 3 h.
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