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. 2019 Mar 22:10:195.
doi: 10.3389/fphar.2019.00195. eCollection 2019.

Resveratrol Ameliorates Glucocorticoid-Induced Bone Damage in a Zebrafish Model

Qun Luo  1   2 , Shengbo Liu  3 , Liming Xie  1 , Yongjie Yu  4 , Limin Zhou  4 , Yuzhen Feng  1 , De Cai  1
Affiliations

Resveratrol Ameliorates Glucocorticoid-Induced Bone Damage in a Zebrafish Model

Qun Luo et al. Front Pharmacol. .

Erratum in

Abstract

Resveratrol (Res) is a multi-functional polyphenol compound that has protective functions in cardiovascular and neurodegenerative diseases. This study aimed to determine the effect of Res on osteogenic differentiation and bone mineralization in zebrafish (Danio rerio) with dexamethasone (Dex)-induced bone damage. Our results showed that Dex exposure (15 μmol/l) decreased the green fluorescence areas and the integrated optic density (IOD) values in the skull bones of zebrafish larvae of the TG(SP7:EGFP) strain in a dose-dependent manner (p < 0.01). Furthermore, Dex exposure decreased the alizarin red S-stained areas (bone mineralization area) in the skeleton and spinal bones of zebrafish larvae of the AB strain in a dose-dependent manner (p < 0.01). By contrast, Res treatment (150 μmol/l) significantly increased both the green fluorescence and bone mineralization area in Dex-exposed zebrafish larvae. Thus, our data show that Res improves bone mineralization after glucocorticoid-induced bone damage in a zebrafish model. Res may be a candidate drug for the prevention of osteoporosis.

Keywords: bone damage; bone mineralization; dexamethasone; resveratrol; zebrafish model.

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Figures

Figure 1
Figure 1
Influence of dexamethasone (Dex) concentration on zebrafish skull. Schematic diagram of TG(SP7:EGFP) zebrafish larvae (A,B). IOD values of green fluorescence of Dex-induced bone damage in TG zebrafish larvae (C). Images of green fluorescence in TG zebrafish larvae skull [in profile (D)]. The Dex concentrations used were 0, 0.25, 5.00, 10.00, 15.00, 20.00, and 25.00 μmol/l, and 0.1% DMSO was used as the control. n = 15, ***p < 0.01.
Figure 2
Figure 2
IC50 and area of bone mineralization after Dex-induced bone damage in AB-strain zebrafish larvae. The 50% inhibitory concentration of dexamethasone (Dex) (A). Analysis of mineralization area in the skull and spine of Dex-treated AB-strain zebrafish larvae at 9 dpf (B). Alizarin red S staining of the skull in Dex-treated AB-strain zebrafish larvae at 9 dpf (C). The Dex concentrations used were 0.25, 1.00, 5.00, 10.00, 15.00, 20.00, and 25.00 μmol/l. The zebrafish larvae were exposed to Dex from 3 to 9 dpf, and 0.1% DMSO served as the control. n = 15, *p < 0.05, ***p < 0.01.
Figure 3
Figure 3
Effect of Res on Dex-induced bone damage in zebrafish. IOD values of green fluorescence of Res after Dex-induced bone damage in TG zebrafish larvae (A). Images of green fluorescence in TG zebrafish larvae skull [in profile (B)]. The Res concentrations used were 25.00, 50.00, 75.00, 100.00, 150.00, 200.00, and 250.00 μmol/l, and 0.1% DMSO served as the control. The area of bone mineralization after Res treatment of Dex-induced bone damage in AB-strain zebrafish larvae at 9 dpf [(C) 15.00 μmol/l Dex vs. 15.00 μmol/l Dex plus 150.00 μmol/l Res]. Alizarin red S staining of the skull after Res treatment of Dex-induced bone damage in AB-strain zebrafish larvae at 9 dpf (D). n = 15, *p < 0.05, ***p < 0.01.
Figure 4
Figure 4
Comparison of survival rates of zebrafish exposed to Dex and Res. Survival rate of zebrafish larvae with Dex-induced bone damage (A). Survival rate of zebrafish larvae with Dex-induced bone damage treated using Res (B). n = 25, *p < 0.05, ***p < 0.01.
Figure 5
Figure 5
The curves of survival rates of zebrafish exposed to Dex and Res. Survival rates of dexamethasone-treated zebrafish (A); Survival rate of zebrafish exposed to resveratrol and dexamethasone (B).
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