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Review
. 2019 Apr 10;7(4):103.
doi: 10.3390/microorganisms7040103.

Functional Genomics of Aspergillus oryzae: Strategies and Progress

Bin He  1 , Yayi Tu  2 , Chunmiao Jiang  3 , Zhe Zhang  4 , Yongkai Li  5 , Bin Zeng  6
Affiliations
Review

Functional Genomics of Aspergillus oryzae: Strategies and Progress

Bin He et al. Microorganisms. .

Abstract

Aspergillus oryzae has been used for the production of traditional fermentation and has promising potential to produce primary and secondary metabolites. Due to the tough cell walls and high drug resistance of A. oryzae, functional genomic characterization studies are relatively limited. The exploitation of selection markers and genetic transformation methods are critical for improving A. oryzae fermentative strains. In this review, we describe the genome sequencing of various A. oryzae strains. Recently developed selection markers and transformation strategies are also described in detail, and the advantages and disadvantages of transformation methods are presented. Lastly, we introduce the recent progress on highlighted topics in A. oryzae functional genomics including conidiation, protein secretion and expression, and secondary metabolites, which will be beneficial for improving the application of A. oryzae to industrial production.

Keywords: Aspergillus oryzae; functional genomics; selection markers; transformation strategies.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Transformation process of A. oryzae using protoplasts. All procedures are performed in the presence of an osmotic stabilizer. DPY: dextrin–peptone–yeast medium. ura: uracil.
Figure 2
Figure 2
Transformation of auxotrophic A. oryzae using the AMT method. IM: induction medium.
See this image and copyright information in PMC

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