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. 1986 Aug;6(8):2807-19.
doi: 10.1128/mcb.6.8.2807-2819.1986.

Features of the pp60v-src carboxyl terminus that are required for transformation

P Yaciuk, D Shalloway

Features of the pp60v-src carboxyl terminus that are required for transformation

P Yaciuk et al. Mol Cell Biol. 1986 Aug.

Abstract

Analysis of the biological and biochemical activities of pp60recombinant-src proteins encoded by 12 carboxyl-terminal mutants showed that a wide family of alternate src carboxyl termini permit complete transforming and kinase activities. src proteins having carboxyl termini which are up to 10 amino acids longer than that of pp60c-src (17 amino acids longer than that of pp60v-src) still permit transformation. Transformation-positive mutations preserve leucine-516, a residue which is highly conserved in protein-tyrosine kinase sequences; removal causes in vivo protein instability. Successive deletion mutants show that this residue is at the boundary of a region required for kinase activity. pp60src which is truncated just outside this point still transforms cells and binds both pp50 and pp90 cellular proteins.

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References

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