Formation of an antioxidant profile in the sea trout (Salmo trutta m. trutta L.) from the Slupia River

Abstract

Using a stage- and sex-based multivariate significance tests on the sea trout Salmo trutta m. trutta L. model, we show dependencies in the balance between lipid peroxidation processes, levels of carbonyl derivatives, and activity of antioxidant enzymes (superoxide dismutase SOD, catalase CAT, glutathione reductase GR, and peroxidase GPx) in the processes of antioxidant profile formation during the fish growing process. The study was aimed at examination of the relationships between the biomarkers of oxidative stress estimated by the total antioxidant status as well as the dependencies between the sex (male, female) and developmental stage of the wild sea trout from the Slupia River and its catchment area rivers. Functioning of the pro/antioxidant balance of the liver tissue reflected the course of the individual developmental stages of the trout and was associated with significant intensification of lipoperoxidation, oxidative modification of proteins, and reduction of the total antioxidant capacity of fish along with age. Formation of a holistic model for the analysis of the involvement of all parameters of antioxidant protection in all stages of development and sex allowed us to obtain the following rank order for the level of lipoperoxidation processes, modified proteins, and antioxidant enzyme complex: CAT > SOD > GPx > GR and TBARS > OMP KD > TAC > OMP AD.

Keywords: antioxidants; developmental stage; oxidative stress; oxidatively modified proteins; sea trout; sex.