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. 2019:1971:123-140.
doi: 10.1007/978-1-4939-9210-2_6.

Cosmid Library Construction and Functional Cloning

Joachim Clos  1 , Dorothea Zander-Dinse  2
Affiliations

Cosmid Library Construction and Functional Cloning

Joachim Clos et al. Methods Mol Biol. 2019.

Abstract

Cosmid libraries can represent an entire genome in a library of circular DNA molecules, allowing for the faithful amplification, cloning and isolation of large genomic DNA fragments. Moreover, using the so-called shuttle cosmid vectors, genomic DNA may be propagated in bacteria and in eukaryotic cells, which is a prerequisite for classic functional cloning and for the newly described Cos-Seq strategies.

Keywords: Cosmid library evaluation; Cosmid library preparation; Functional cloning; High-quality gDNA extraction.

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