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. 2019 Apr 3;24(7):1317.
doi: 10.3390/molecules24071317.

A Study of 5-Fluorouracil Desorption from Mesoporous Silica by RP-UHPLC

Monika Šuleková  1 , Lucia Váhovská  2 , Alexander Hudák  3 , Lukáš Žid  4 , Vladimír Zeleňák  5
Affiliations

A Study of 5-Fluorouracil Desorption from Mesoporous Silica by RP-UHPLC

Monika Šuleková et al. Molecules. .

Abstract

In cancer treatment, the safe delivery of the drug to the target tissue is an important task. 5-fluorouracil (5-FU), the well-known anticancer drug, was encapsulated into the pores of unmodified mesoporous silica SBA-15, as well as silica modified with 3-aminopropyl and cyclohexyl groups. The drug release studies were performed in two different media, in a simulated gastric fluid (pH = 2) and in a simulated body fluid (pH = 7) by RP-UHPLC. The simple and rapid RP-UHPLC method for quantitative determination of 5-fluorouracil released from unmodified and modified mesoporous silica SBA-15 was established on ODS Hypersil C18 column (150 × 4.6 mm, 5 µm) eluted with mobile phase consisted of methanol: phosphate buffer in volume ratio of 3:97 (v/v). Separation was achieved by isocratic elution. The flow rate was kept at 1 mL/min, the injection volume was set at 20 µL and the column oven temperature was maintained at 25 °C. The effluent was monitored at 268 nm. This paper provides information about the quantitative determination of the released 5-FU from silica. It was found out that larger amount of the drug was released in neutral pH in comparison with the acidic medium. In addition, surface functionalisation of silica SBA-15 influences the release properties of the drug.

Keywords: 5-fluorouracil; RP-UHPLC; desorption; mesoporous silica.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Chemical structure of 5-fluorouracil.
Figure 2
Figure 2
Isotherms of mesoporous silica SBA-15 before (blue) and after modification with 3-aminopropyl (cyan) and cyclohexyl (green) groups.
Figure 3
Figure 3
BJH pore size distribution for nanomaterials SBA-15 (A), nSBA-15 (B) and cSBA-15 (C) shows decrease in radius after modification step.
Figure 4
Figure 4
Schematic representation of possible reason for lower number of cyclohexyl groups in comparison to 3-aminopropyl groups. In the process of modification (A) the methanol was released as a by-product (B) and slowly diffuse into the reaction media. Alcohols can bind to the MPS surface and modify it (C), which results in the lower number of free hydroxyl groups.
Figure 5
Figure 5
HRTEM micrograph of SBA-15.
Figure 6
Figure 6
XRD pattern of samples SBA-15 (black) and SBA-15 with 5-fluorouracil loaded (red).
Figure 7
Figure 7
Infrared spectra of nanomaterials SBA-15 (A), 5-FU (B), 5-FU-SBA-15 (C), nSBA-15 (D), 5-FU-nSBA-15 (E), cSBA-15 (F) and 5-FU-cSBA-15 (G).
Figure 7
Figure 7
Infrared spectra of nanomaterials SBA-15 (A), 5-FU (B), 5-FU-SBA-15 (C), nSBA-15 (D), 5-FU-nSBA-15 (E), cSBA-15 (F) and 5-FU-cSBA-15 (G).
Figure 8
Figure 8
Absorption spectrum of 5-FU.
Figure 9
Figure 9
Chromatogram of 5-FU standard solution (25 µg/mL) and calibration curve for 5-FU.
Figure 10
Figure 10
Chemical structure of the 3-aminopropyl (left) and cyclohexyl (right) groups.
Figure 11
Figure 11
Representative chromatogram of 5-FU released during 4 h from SBA-15 into medium with pH = 2 (left) and medium with pH = 7 (right).
Figure 12
Figure 12
Release profiles of 5-fluorouracil from 5-FU-SBA-15 (A), 5-FU-nSBA-15 (B) and 5-FU-cSBA-15 (C) nanomaterials into the media with different pH.
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References

    1. Yang P., Gai S., Lin J. Functionalized mesoporous silica materials for controlled drug delivery. Chem. Soc. Rev. 2012;41:3679–3698. doi: 10.1039/c2cs15308d. - DOI - PubMed
    1. Böttcher H., Slowik P., Süβ W. Sol-Gel Carrier Systems for Controlled Drug Delivery. J. Sol–Gel Sci. Technol. 1998;13:277. doi: 10.1023/A:1008603622543. - DOI
    1. Wu Z., Joo H., Lee T.G., Lee K. Controlled release of lidocaine hydrochloride from the surfactant-doped hybrid xerogels. J. Control. Release. 2005;104:497. doi: 10.1016/j.jconrel.2005.02.023. - DOI - PubMed
    1. Manzano M., Vallet-Regí M. New developments in ordered mesoporous materials for drug delivery. J. Mater. Chem. 2010;20:5593–5604. doi: 10.1039/b922651f. - DOI
    1. Zhao D., Feng J., Huo Q., Melosh N., Fredrickson G.H., Chmelka B.F., Stucky G.D. Triblock copolymer syntheses of mesoporous silica with periodic 50 to 300 angstrom pores. Science. 1998;279:548–552. doi: 10.1126/science.279.5350.548. - DOI - PubMed

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