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. 1987 Jan;25(1):76-80.
doi: 10.1128/jcm.25.1.76-80.1987.

Production, characterization, and species specificity of five monoclonal antibodies to Mycobacterium tuberculosis

G V Kadival, S D Chaparas

Production, characterization, and species specificity of five monoclonal antibodies to Mycobacterium tuberculosis

G V Kadival et al. J Clin Microbiol. 1987 Jan.

Abstract

The production and characterization of five monoclonal antibodies to Mycobacterium tuberculosis are described. Specificity of the monoclonal antibodies was tested against other mycobacterial species by an enzyme-linked immunosorbent assay and immunoblots. HGT 3a, an immunoglobulin M (IgM) antibody, recognizes a molecule of 38,000 molecular weight present only in the tuberculosis complex of M. tuberculosis and Mycobacterium bovis BCG. HGT 6, an IgG1 antibody, recognized two molecules with molecular weights of 43,000 and 45,000 and showed limited cross-reactivity. Three other antibodies, HGT 1, HGT 2, and HGT 4, all belonging to the IgG1 type, recognized multiple bands and showed broad reactivity among all mycobacterial antigens tested, Escherichia coli and Nocardia asteroides.

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References

    1. J Mol Biol. 1971 Dec 28;62(3):465-77 - PubMed
    1. Nature. 1975 Aug 7;256(5517):495-7 - PubMed
    1. Am Rev Respir Dis. 1978 Jun;117(6):1091-7 - PubMed
    1. Lancet. 1981 Jul 25;2(8239):167-9 - PubMed
    1. Infect Immun. 1982 Jul;37(1):172-8 - PubMed

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