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. 2019 May;17(5):3664-3670.
doi: 10.3892/etm.2019.7341. Epub 2019 Mar 4.

Effects of curcumin on artery blood gas index of rats with pulmonary fibrosis caused by paraquat poisoning and the expression of Smad 4, Smurf 2, interleukin-4 and interferon-γ

Honggang Chen  1 , Rongjia Yang  1 , Yan Tang  2 , Xiaoyan Fu  3
Affiliations

Effects of curcumin on artery blood gas index of rats with pulmonary fibrosis caused by paraquat poisoning and the expression of Smad 4, Smurf 2, interleukin-4 and interferon-γ

Honggang Chen et al. Exp Ther Med. 2019 May.

Abstract

Effects of curcumin on artery blood gas index of rats with pulmonary fibrosis caused by paraquat poisoning and the expression of Mothers against decapentaplegic homolog 4 (Smad4), Smad ubiquitination regulatory factor 2 (Smurf2), interleukin-4 (IL-4) and interferon-γ (IFN-γ) were explored. A total of 54 Wistar rats were randomly selected, of which 36 rats were selected for paraquat poisoning pulmonary fibrosis modeling, and 18 were used in the control group for normal feeding. Then, 18 rats were randomly selected from the modeled groups and injected with curcumin and classified as the curcumin group. The remaining 18 rats were not processed and 17 were successfully modeled as the paraquat group. The expression of SMAD4, Smurf2, IL-4 and INF-γ was detected by enzyme-linked immunosorbent assay. Abdominal aortic blood was taken for determination of pH, arterial partial pressure of oxygen (PaO2) and arterial partial pressure of carbon dioxide (PaCO2). The artery blood PaO2 and serum INF-γ of the curcumin and paraquat groups were significantly higher on day 1 than those on day 5 (P<0.05). The artery blood PaO2 and serum INF-γ in the curcumin group were higher than those in the paraquat group (P<0.05). The artery blood PaCO2, serum Smad4, Smurf2 and IL-4 in the curcumin group were significantly lower on day 1 than those on day 5 (P<0.05). The artery blood PaCO2, serum Smad4, Smurf2 and IL-4 in the paraquat group were significantly lower on day 1 than those on day 5 (P<0.05). The PaCO2, serum Smad4, Smurf2 and IL-4 in the curcumin group were lower than those in the paraquat group (P<0.05). In conclusion, curcumin can effectively improve pulmonary fibrosis in rats after treatment with paraquat poisoning. The results show that it is expected to be an effective drug for the treatment of paraquat, and provide effective reference and guidance for clinical treatment.

Keywords: curcumin; paraquat poisoning; pulmonary fibrosis; rat model.

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Figures

Figure 1.
Figure 1.
Comparison of artery blood pH values of rats between the three groups. The pH values of artery blood on days 1 and 5 in curcumin group were 7.37±0.08 and 7.39±0.06, respectively. The pH values of artery blood on days 1 and 5 in paraquat group were 7.40±0.04 and 7.41±0.02, respectively. The pH values of artery blood on days 1 and 5 in the control group were 7.37±0.06 and 7.38±0.03, respectively. There was no significant difference in the pH value of artery blood between days 1 and 5 in the curcumin group, the paraquat group and the control group (P>0.05). On days 1 and 5, the artery blood pH values of the three groups was measured, and there was no significant difference and was not statistically significant (P>0.05).
Figure 2.
Figure 2.
Comparison of PaO2 in artery blood of rats between the three groups. The PaO2 of artery blood on days 1 and 5 in the curcumin group was 83.75±13.65 and 67.39±15.57 mmHg, respectively. The PaO2 of artery blood on days 1 and 5 in the paraquat group was 76.37±13.19 and 50.40±16.53 mmHg, respectively, the artery blood PaO2 of the control group on days 1 and 5 were 89.65±16.43 and 87.38±12.34 mmHg, respectively. PaO2 in artery blood of rats in the curcumin group on day 1 was significantly higher than that on day 5, which was statistically significant (P<0.05). PaO2 in artery blood of rats in the paraquat group was significantly higher on day 1 than that on day 5, which was statistically significant (P<0.001). There was no significant difference in PaO2 of artery blood on days 1 and 5 in the control group, which was not statistically significant (P>0.05). On days 1 and 5, PaO2 in the artery blood of rats between the three groups was statistically significant (P<0.001). The artery blood PaO2 of the control group was higher than that in the curcumin group and paraquat group, which were statistically significant (P<0.05). The PaO2 of artery blood in the curcumin group was higher than that in the paraquat group, which was statistically significant (P<0.05). *P<0.05, when compared with the curcumin group; #P<0.05, when compared with the paraquat group and ^P<0.05, when compared to day 1.
Figure 3.
Figure 3.
Comparison of artery blood PaCO2 values of rats between the three groups. The PaCO2 of artery whole blood on days 1 and 5 in the curcumin group were 40.41±5.16 mmHg and 53.73±5.72 mmHg, respectively. The PaCO2 of artery blood on days 1 and 5 in the paraquat group was 43.56±6.39 mmHg and 69.67±6.53 mmHg, respectively. The PaCO2 of artery blood days 1 and 5 in the control group was 35.48±5.92 mmHg and 36.54±5.61 mmHg, respectively. PaCO2 in artery blood of rats in the curcumin group was significantly lower on day 1 than that on day 5, which was statistically significant (P<0.001). PaCO2 in artery blood of rats in the paraquat group was significantly lower on day 1 than that on day 5, which was statistically significant (P<0.001); There was no significant difference in PaCO2 on days 1 and 5 in the control group, which was not statistically significant (P>0.05). On days 1 and 5, the artery blood PaCO2 of rats between the three groups was statistically significant (P<0.001); The artery blood PaCO2 of the control group was lower than that in the curcumin group and paraquat group, which were statistically significant (P<0.05). The PaCO2 of artery blood in the curcumin group was lower than that in the paraquat group, which was statistically significant (P<0.05). *P<0.05, when compared with the curcumin group; #P<0.05, when compared with the paraquat group and ^P<0.05, compared to day 1.
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