Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Mar 18;16(2):161-175.
doi: 10.1007/s13770-019-00185-z. eCollection 2019 Apr.

Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films

Iro Koliakou #  1   2 , Eleni Gounari #  2   3 , Maria Nerantzaki  4   5 , Eleni Pavlidou  6 , Dimitrios Bikiaris  4 , Martha Kaloyianni  1 , George Koliakos  2   3
Affiliations

Differentiation Capacity of Monocyte-Derived Multipotential Cells on Nanocomposite Poly(e-caprolactone)-Based Thin Films

Iro Koliakou et al. Tissue Eng Regen Med. .

Abstract

Background: Μonocyte-derived multipotential cells (MOMCs) include progenitors capable of differentiation into multiple cell lineages and thus represent an ideal autologous transplantable cell source for regenerative medicine. In this study, we cultured MOMCs, generated from mononuclear cells of peripheral blood, on the surface of nanocomposite thin films.

Methods: For this purpose, nanocomposite Poly(e-caprolactone) (PCL)-based thin films containing either 2.5 wt% silica nanotubes (SiO2ntbs) or strontium hydroxyapatite nanorods (SrHAnrds), were prepared using the spin-coating method. The induced differentiation capacity of MOMCs, towards bone and endothelium, was estimated using flow cytometry, real-time polymerase chain reaction, scanning electron microscopy and fluorescence microscopy after cells' genetic modification using the Sleeping Beauty Transposon System aiming their observation onto the scaffolds. Moreover, Wharton's Jelly Mesenchymal Stromal Cells were cultivated as a control cell line, while Human Umbilical Vein Endothelial Cells were used to strengthen and accelerate the differentiation procedure in semi-permeable culture systems. Finally, the cytotoxicity of the studied materials was checked with MTT assay.

Results: The highest differentiation capacity of MOMCs was observed on PCL/SiO2ntbs 2.5 wt% nanocomposite film, as they progressively lost their native markers and gained endothelial lineage, in both protein and transcriptional level. In addition, the presence of SrHAnrds in the PCL matrix triggered processes related to osteoblast bone formation.

Conclusion: To conclude, the differentiation of MOMCs was selectively guided by incorporating SiO2ntbs or SrHAnrds into a polymeric matrix, for the first time.

Keywords: Monocyte-derived multipotential cells; Poly(ε-caprolactone); Silica nanotubes; Strontium hydroxyapatite nanorods.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.This study was approved by the Ethics Committee of Aristotle University of Thessaloniki, School of Medicine (390-9/1.7.2017) and University Hospital AXEPA of Thessaloniki (3868/24.1.2018). This research involves Human Participants after their informed consent. Peripheral blood samples were collected from healthy volunteer donors while mesenchymal stromal cells and HUVEC were isolated from Wharton’s jelly after parents approval during stem cell banking in Biohellenika SA Biotechnology Company.

Figures

Fig. 1
Fig. 1
A Morphology of genetically modified normal WJSCs (a, b) and HUVEC (c, d) in cell culture. B Immunophenotypic characterization of WJSCs (a, b) and HUVEC (c) via flow cytometry
Fig. 2
Fig. 2
A Comparative MOMCs culture in the presence of poly-l-lysine (upper) and fibronectin (below). B Immunophenotypic characterization of MOMCs after culture in the presence of poly-l-lysine and medium supplemented with SDF-1a
Fig. 3
Fig. 3
A Testing of the NPs-mediated cytotoxicity in WJSCs cultures with MTT assay (a) 24 h and (b) 72 h after their addition in growth medium. B FTIR spectra of (a) PCL/SiO2ntbs 2.5 wt% and (b), PCL/SrHAnrds 2.5 wt% in comparison to the spectra of their pure components
Fig. 4
Fig. 4
Observation of morphological differentiation of A WJSCs and B MOMCs upon endothelial differentiation on different NP category PCL films
Fig. 5
Fig. 5
A Immunophenotypic evaluation of the WJSCs (left) and MOMCs (right) differentiation towards endothelial cells. B Flow cytometry images of the CD45/CD144 marker expression before and after the completion of the WJSCs and MOMCs differentiation towards endothelium upon PCL/SiO2ntbs films
Fig. 6
Fig. 6
Real-time PCR for the evaluation of MOMCs and WJSCs differentiation in transcriptional level
Fig. 7
Fig. 7
A, B SEM depiction of the WJSCs and MOMCs before and after their differentiation upon PCL films
Fig. 8
Fig. 8
Evaluation of the differentiation capacity towards bone cells with the CPC method for MOMCS as well as WJSCs
See this image and copyright information in PMC

References

    1. Dawson E, Mapili G, Erickson K, Taqvi S, Roy K. Biomaterials for stem cell differentiation. Adv Drug Deliv Rev. 2008;60:215–228. doi: 10.1016/j.addr.2007.08.037. - DOI - PubMed
    1. Keane TJ, Badylak SF. Biomaterials for tissue engineering applications. Semin Pediatr Surg. 2014;23:112–118. doi: 10.1053/j.sempedsurg.2014.06.010. - DOI - PubMed
    1. O’Brien FJ. Biomaterials & scaffolds for tissue engineering. Mater Today (Kidlington) 2011;14:88–95. doi: 10.1016/S1369-7021(11)70058-X. - DOI
    1. Vacanti J. Tissue engineering and regenerative medicine: from first principles to state of the art. J Pediatr Surg. 2010;45:291–294. doi: 10.1016/j.jpedsurg.2009.10.063. - DOI - PubMed
    1. Howard D, Buttery LD, Shakesheff KM, Roberts SJ. Tissue engineering: strategies, stem cells and scaffolds. J Anat. 2008;213:66–72. doi: 10.1111/j.1469-7580.2008.00878.x. - DOI - PMC - PubMed

MeSH terms

LinkOut - more resources