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. 1986 Dec;6(12):4763-6.
doi: 10.1128/mcb.6.12.4763-4766.1986.

DNA binding is not sufficient for nuclear localization of regulatory proteins in Saccharomyces cerevisiae

P A Silver, R Brent, M Ptashne

DNA binding is not sufficient for nuclear localization of regulatory proteins in Saccharomyces cerevisiae

P A Silver et al. Mol Cell Biol. 1986 Dec.

Abstract

We showed by immunofluorescence that the procaryotic DNA-binding protein LexA and a chimeric protein that contains the DNA-binding portion of LexA (amino acids 1 to 87) and a large portion (amino acids 74 to 881) of the Saccharomyces cerevisiae positive regulatory GAL4 protein (GAL4 gene product) are not preferentially localized in the nucleus in S. cerevisiae.

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References

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