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. 1978 Dec 1;148(6):1612-9.
doi: 10.1084/jem.148.6.1612.

B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay

M H Schreier

B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay

M H Schreier. J Exp Med. .

Abstract

A sensitive, specific, and reproducible in vitro helper assay is described which is suited to limiting dilution analysis of murine B cells. 1 in about 3,000 syngeneic splenic B cells can be induced to form plaque-forming cells (PFC) to sheep erythrocytes in this system. The induction of PFC is absolutely dependent on antigen and specific helper T cells.

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