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Comment
. 2019 Apr 12:4:9.
doi: 10.1038/s41392-019-0044-y. eCollection 2019.

Off-Targeting of Base Editors: BE3 but not ABE induces substantial off-target single nucleotide variants

Huhu Xin  1 , Tao Wan  1 , Yuan Ping  1
Affiliations
Comment

Off-Targeting of Base Editors: BE3 but not ABE induces substantial off-target single nucleotide variants

Huhu Xin et al. Signal Transduct Target Ther. .
No abstract available

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Figures

Fig. 1
Fig. 1
Experimental designs of Yang’s (a) and Gao’s (b) works. a Ai9 is a transgene mouse strain that can express robust tdTomato fluorescence following Cre-mediated recombination. A mixture of Cas9 mRNA, BE3 mRNA or ABE7.10 mRNA, sgRNA and Cre mRNA was injected into one blastomere of a 2-cell embryo. The tdTomato+ and tdTomato- cells from E14.5 embryos were isolated by flow cytometry (FACS). Then, the two groups of cells were sequenced by WGS (whole genome sequencing) and analyzed by different algorithms. b Plant expression vectors of BE3, high-fidelity BE3 (HFI-BE3) or ABE were introduced into rice callus cells by Agrobacterium-mediated transformation, and then the base-edited plants and control plants were examined, similar to Yang’s work
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References

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