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. 2019:27:e20180205.
doi: 10.1590/1678-7757-2018-0205. Epub 2019 Apr 11.

Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy

Mariana Gouvêa Latini Abreu  1 , Dione Kawamoto  2 , Marcia Pinto Alves Mayer  2 , Vinicius D'Avila Bitencourt Pascoal  3 , Karina Sampaio Caiaffa  4 , Elizangela P Zuza  1 , Cristiane Duque  5 , Gabriela Alessandra da Cruz Galhardo Camargo  1
Affiliations

Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy

Mariana Gouvêa Latini Abreu et al. J Appl Oral Sci. 2019.

Abstract

Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium.

Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy.

Material and methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction.

Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%).

Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.

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Figures

Figure 1
Figure 1. Frequency (%) of combinations of P. gingivalis fimA genes in smokers (SM) and nonsmokers (NS) at baseline and after 3 months of periodontal treatment. Values express percentage of P. gingivalis harboring fimA types
See this image and copyright information in PMC

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