Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Apr 18:25:2845-2851.
doi: 10.12659/MSM.913359.

Long Non-Coding RNA BANCR Is Overexpressed in Patients with Diabetic Retinopathy and Promotes Apoptosis of Retinal Pigment Epithelial Cells

Li Yin  1 , Zhaohui Sun  1 , Qian Ren  1 , Xian Su  1 , Delong Zhang  1
Affiliations

Long Non-Coding RNA BANCR Is Overexpressed in Patients with Diabetic Retinopathy and Promotes Apoptosis of Retinal Pigment Epithelial Cells

Li Yin et al. Med Sci Monit. .

Abstract

BACKGROUND lncRNA BANCR participates in the pathogenesis of various types of human diseases; however, its involvement in diabetic retinopathy is unknown. MATERIAL AND METHODS In this study, the expression of lncRNA BANCR in plasma of patients with diabetic retinopathy, diabetic patients without complications, and healthy controls was analyzed by qRT-PCR. The accuracy plasma BANCR in diagnosing diabetic retinopathy was analyzed by ROC curve analysis. lncRNA BANCR expression vector and siRNA were transfected into the human retinal pigment epithelial cell line ARPE-19, and cell apoptosis was analyzed by cell apoptosis assay. RESULTS We found that lncRNA BANCR was significantly upregulated in patients with diabetic retinopathy compared to diabetic patients without complications and healthy controls. Upregulation of lncRNA BANCR effectively distinguished patients with diabetic retinopathy from diabetic patients without complications and healthy controls. High-glucose treatment led to upregulated expression of BANCR in the human retinal pigment epithelial cell line ARPE-19. Overexpression of BANCR promoted and siRNA silencing inhibited the apoptosis of cells in the human retinal pigment epithelial cell line ARPE-19 in a high-glucose environment. CONCLUSIONS lncRNA BANCR is overexpressed in patients with diabetic retinopathy and can promote apoptosis of retinal pigment epithelial cells.

PubMed Disclaimer

Figures

Figure 1
Figure 1
lncRNA BANCR was significantly upregulated in diabetic retinopathy patients. Compared with the diabetes and control groups, significantly upregulated expression levels of lncRNA BANCR were observed in plasma of patients with diabetic retinopathy (* p<0.05).
Figure 2
Figure 2
Overexpression of lncRNA BANCR has diagnostic potentials for diabetic retinopathy. This figure shows the ROC curve analysis of the diagnostic value of lncRNA BANCR for diabetic retinopathy with diabetic patients (A) or healthy controls (B) as true-negative subjects, and the diagnostic value of lncRNA BANCR for diabetes with healthy controls as true-negative subjects (C).
Figure 3
Figure 3
High-glucose environment upregulated the expression of lncRNA BANCR. A high-glucose environment led to upregulated expression of lncRNA BANCR in a dose- and time-dependent manner (* p<0.05).
Figure 4
Figure 4
lncRNA BANCR overexpression promoted (A), while BANCR siRNA silencing inhibited (B), the apoptosis of ARPE-19 cells (* p<0.05).
See this image and copyright information in PMC

References

    1. Nentwich MM, Ulbig MW. Diabetic retinopathy-ocular complications of diabetes mellitus. World J Diabetes. 2015;6(3):489–99. - PMC - PubMed
    1. Thomas RL, Dunstan FD, Luzio SD, et al. Prevalence of diabetic retinopathy within a national diabetic retinopathy screening service. Br J Ophthalmol. 2015;99(1):64–68. - PubMed
    1. Foster A. Planning a programme to prevent visual loss from diabetic retinopathy. Community Eye Health. 2015;28(92):s1–5. - PMC - PubMed
    1. NCD Risk Factor Collaboration (NCD-RisC) Worldwide trends in diabetes since 1980: A pooled analysis of 751 population-based studies with 4.4 million participants. Lancet. 2016;387(10027):1513–30. - PMC - PubMed
    1. Menke A, Casagrande S, Geiss L, et al. Prevalence of and trends in diabetes among adults in the United States, 1988–2012. JAMA. 2015;314(10):1021–29. - PubMed

MeSH terms