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. 2019 May 1;95(5):fiz045.
doi: 10.1093/femsec/fiz045.

Issues and current standards of controls in microbiome research

Bastian V H Hornung  1   2 , Romy D Zwittink  1   2 , Ed J Kuijper  1   2   3
Affiliations

Issues and current standards of controls in microbiome research

Bastian V H Hornung et al. FEMS Microbiol Ecol. .

Abstract

Good scientific practice is important in all areas of science. In recent years this has gained more and more attention, especially considering the 'scientific reproducibility crisis'. While most researchers are aware of the issues with good scientific practice, not all of these issues are necessarily clear, and the details can be very complicated. For many years it has been accepted to perform and publish sequencing based microbiome studies without including proper controls. Although in recent years more scientists realize the necessity of implementing controls, this poses a problem due to the complexity of the field. Another concern is the inability to properly interpret the information gained from controls in microbiome studies. Here, we will discuss these issues and provide a comprehensive overview of problematic points regarding controls in microbiome research, and of the current standards in this area.

Keywords: best practices; contamination; metagenomics; microbiome; microbiota; negative control; positive control.

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