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. 2019 Apr 17;24(8):1521.
doi: 10.3390/molecules24081521.

Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

Yehong Li  1 , Yiming Zhang  2 , Zejun Zhang  3 , Yupiao Hu  4 , Xiuming Cui  5   6   7 , Yin Xiong  8   9   10
Affiliations

Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

Yehong Li et al. Molecules. .

Abstract

Gastrodia elata (G. elata) tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for G. elata tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of G. elata has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) (p > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of G. elata tuber and giving references to promote the quality standards of herbal medicines.

Keywords: Gastrodia elata tuber; HPLC; QAMS; quality evaluation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The HPLC fingerprints of the Gastrodia elata tuber sample and the mixed standards. R: The mixed standards; S: The G. elata tuber sample. 1—Gastrodin; 2—p-Hydroxy benzyl alcohol; 3—Parishin E; 4—p-Hydroxy benzaldehyde; 5—Parishin B; 6—Parishin C; 7—Parishin A.
Figure 2
Figure 2
HPLC fingerprints of the 21 batches of G. elata tuber samples. 1—Gastrodin; 2—p-Hydroxy benzyl alcohol; 3—Parishin E; 4—p-Hydroxy benzaldehyde; 5—Parishin B; 6—Parishin C; 7—Parishin A.
Figure 3
Figure 3
Clustering analysis graph of the 21 G. elata tuber samples.
Figure 4
Figure 4
The structures of some compounds in the G. elata tuber. (a) Gastrodin [34], (b) p-hydroxy benzaldehyde [35], and (c) p-hydroxybenzyl alcohol [36].
Figure 5
Figure 5
The structures of parishins in the G. elata tuber. The structure of parishins [13]: RA. parishin A, RB. parishin B, RC. parishin C, RE. parishin E.
Figure 5
Figure 5
The structures of parishins in the G. elata tuber. The structure of parishins [13]: RA. parishin A, RB. parishin B, RC. parishin C, RE. parishin E.
See this image and copyright information in PMC

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