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. 2019 Apr 18;19(1):60.
doi: 10.1186/s12903-019-0747-z.

Impact of polyols on Oral microbiome of Estonian schoolchildren

Jelena Štšepetova  1   2 , Jaak Truu  3 , Riina Runnel  4 , Rita Nõmmela  4 , Mare Saag  4 , Jana Olak  4 , Hiie Nõlvak  3 , Jens-Konrad Preem  3 , Kristjan Oopkaup  3 , Kaarel Krjutškov  5 , Eino Honkala  6 , Sisko Honkala  6 , Kauko Mäkinen  7 , Pirkko-Liisa Mäkinen  7 , Tero Vahlberg  8 , Joan Vermeiren  9 , Douwina Bosscher  9 , Peter de Cock  9 , Reet Mändar  10   11
Affiliations

Impact of polyols on Oral microbiome of Estonian schoolchildren

Jelena Štšepetova et al. BMC Oral Health. .

Abstract

Background: Oral microbiome has significant impact on both oral and general health. Polyols have been promoted as sugar substitutes in prevention of oral diseases. We aimed to reveal the effect of candies containing erythritol, xylitol or control (sorbitol) on salivary microbiome.

Methods: Ninety children (11.3 ± 0.6 years) consumed candies during 3 years. Microbial communities were profiled using Illumina HiSeq 2000 sequencing and real-time PCR.

Results: The dominant phyla in saliva were Firmicutes (39.1%), Proteobacteria (26.1%), Bacteroidetes (14.7%), Actinobacteria (12%) and Fusobacteria (6%). The microbiome of erythritol group significantly differed from that of the other groups. Both erythritol and xylitol reduced the number of observed bacterial phylotypes in comparison to the control group. The relative abundance of the genera Veillonella, Streptococcus and Fusobacterium were higher while that of Bergeyella lower after erythritol intervention when comparing with control. The lowest prevalence of caries-related mutans streptococci corresponded with the lowest clinical caries markers in the erythritol group.

Conclusions: Daily consumption of erythritol, xylitol or control candies has a specific influence on the salivary microbiome composition in schoolchildren. Erythritol is associated with the lowest prevalence of caries-related mutans streptococci and the lowest levels of clinical caries experience.

Trial registration: ClinicalTrials.gov Identifier NCT01062633.

Keywords: Erythritol; Next generation sequencing; Oral microbiome; Polyol; Saliva; qPCR.

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Conflict of interest statement

Ethics approval and consent to participate

The study was performed according to Declaration of Helsinki and approved by the Ethics Review Committee on Human Research of the University of Tartu (no. 166/7 17.12.2007). The written agreement forms were signed by parents or caretakers of the participants.

Consent for publication

Not applicable.

Competing interests

Joan Vermeiren, Douwina Bosscher and Peter de Cock were employed by Cargill during the preparation of this manuscript, and Cargill produces erythritol. No potential conflict of interest was reported by other authors.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Relative abundance of bacterial phyla (a) and families (b) within studied groups. Groups: ery – erythritol, sorb – sorbitol, xyl – xylitol
Fig. 2
Fig. 2
Heat map showing the relative abundance of the most predominant genera in saliva. Row represents the relative percentage of each bacterial genus, and column stands for different samples
Fig. 3
Fig. 3
Plot of principal component analysis based on Hellinger transformed OTU relative abundance values. Individual saliva microbiome samples are connected to treatment centroids. First and second principal axes describe 16.7 and 13.3% of overall variation, respectively. Abbreviations: ery - erythritol, sorb - sorbitol, xyl - xylitol
See this image and copyright information in PMC

References

    1. Paster BJ, Olsen I, Aas JA, Dewhirst FE. The breadth of bacterial diversity in the human periodontal pockets and other oral sites. Periodontol. 2006;42:80–87. doi: 10.1111/j.1600-0757.2006.00174.x. - DOI - PubMed
    1. Keijser BJ, Zaura E, Huse SM, et al. Pyrosequencing analysis of the oral microflora of healthy adults. J Dent Res. 2008;87:1016–1020. doi: 10.1177/154405910808701104. - DOI - PubMed
    1. Wade WG. The oral microbiome in health and disease. Pharmacol Res. 2013;69:137–143. doi: 10.1016/j.phrs.2012.11.006. - DOI - PubMed
    1. Zaura E, Keijser BJ, Huse SM, Crielaard W. Defining the healthy ‘core microbiome’ of the oral microbial communities. BMC Microbiol. 2009;9:259–270. doi: 10.1186/1471-2180-9-259. - DOI - PMC - PubMed
    1. Dewhirst FE, Chen T, Izard J, et al. The human oral microbiome. J Bacteriol. 2010;192:5002–5007. doi: 10.1128/JB.00542-10. - DOI - PMC - PubMed

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