Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase
- PMID: 31001398
- PMCID: PMC6461724
- DOI: 10.4317/jced.55546
Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase
Abstract
Background: The biological responses involved during retention phase have been studied for many years but little is known about the effect of saliva proteome during retention phase of post-orthodontic treatment. This study aims to identify the protein profiles during retention phase in relation to biological processes involved by Liquid Chromatography Mass Spectrometry (LC-MS) approach.
Material and methods: A total of 5 ml of unstimulated saliva was collected from each subject (10 non-orthodontic patients and 15 post-orthodontic patients with 6-months retention phase). Samples were then subjected to LC-MS analysis. The expressed proteins were identified and compared between groups. Incisor irregularity for both maxilla and mandible were determined with Little's Irregularity Index at 6-months retention phase.
Results: 146 proteins and 135 proteins were expressed in control and 6-months retention phase group respectively. 15 proteins were identified to be co-expressed between groups. Immune system process was only detected in 6-months retention phase group. Detected protein in immune system process was identified as Tyrosine-protein kinase Tec. Statistical significant of incisor irregularity was only found in mandible at 6-months retention phase.
Conclusions: Our study suggests that immune system process protein which is Tyrosine-protein kinase Tec could be used as biomarker for prediction of stability during retention phase of post-orthodontic treatment. Key words:Orthodontics, proteomics, retention, LC-MS, saliva.
Conflict of interest statement
Conflict of interest statement:The authors have declared no conflict of interest.
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References
-
- Årtun J, Garol JD, Little RM. Long term stability of mandibular incisors following successful treatment of Class ll Divison 1 malocclusion. Angle Orthodontist. 1996;66:229–238. - PubMed
-
- Francisconi MF, Janson G, Freitas KMS, Oliveira RCG, De Oliveira RCG, De Freitas MR. Overjet, overbite, and anterior crowding relapses in extraction and nonextraction patients, and their correlations. American Journal of Orthodontics and Dentofacial Orthopedics. 2014;146:67–72. - PubMed
-
- Littlewood SJ, Kandasamy S, Huang G. Retention and relapse in clinical practice. Australian Dental Journal. 2017;62:51–57. - PubMed
-
- Little RM, Riedel RA, Stein A, Angle E. Mandibular arch length increase during the mixed dentition: Postretention evaluation of stability and relapse. American Journal of Orthodontics and Dentofacial Orthopedics. 1990;97:393–404. - PubMed
-
- Bondemark L, Holm AK, Hansen K, Axelsson S, Mohlin B, Brattstrom V. Long-term stability of orthodontic treatment and patient satisfaction. The Angle Orthodontist. 2007;77:181–191. - PubMed
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