doi: 10.1016/0014-5793(87)81566-1.
Clostridium botulinum type C produces a novel ADP-ribosyltransferase distinct from botulinum C2 toxin
- PMID: 3100333
- DOI: 10.1016/0014-5793(87)81566-1
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Clostridium botulinum type C produces a novel ADP-ribosyltransferase distinct from botulinum C2 toxin
FEBS Lett.
.
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Abstract
The culture medium of certain strains of Clostridium botulinum type C contains two separable ADP-ribosyltransferases. Besides the ADP-ribosylation of actin due to botulinum C2 I toxin, a second microbial enzyme causes the mono-ADP-ribosylation of a eukaryotic protein with a molecular mass of about 20 kDa found in platelets, neuroblastoma X glioma hybrid cells, S49 lymphoma cells, chick embryo fibroblasts and sperm. The eukaryotic substrate is inactivated by heating and trypsin treatment. In contrast, the novel ADP-ribosyltransferase, which can be separated by DEAE-Sephadex chromatography, is largely resistant in the short term to trypsin digestion.
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