Characterizing the protein corona of sub-10 nm nanoparticles

Dylan Glancy¹, Yuwei Zhang², Jamie L Y Wu³, Ben Ouyang³, Seiichi Ohta⁴, Warren C W Chan⁵

Affiliations

¹ Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada; Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada.
² Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada; Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, Toronto, ON M5S 3G9, Canada.
³ Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, Toronto, ON M5S 3G9, Canada.
⁴ Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, Toronto, ON M5S 3G9, Canada; Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-8655, Japan.
⁵ Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada; Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, Toronto, ON M5S 3G9, Canada; Department of Chemical Engineering, University of Toronto, 200 College Street, Toronto, ON M5S 3E5, Canada; Department of Material Science and Engineering, University of Toronto, 160 College Street, Room 450, Toronto M5S 3E1, Canada. Electronic address: warren.chan@utoronto.ca.

PMID: 31004667
DOI: 10.1016/j.jconrel.2019.04.023

Characterizing the protein corona of sub-10 nm nanoparticles

Dylan Glancy et al. J Control Release. 2019.

. 2019 Jun 28:304:102-110.

doi: 10.1016/j.jconrel.2019.04.023. Epub 2019 Apr 17.

Authors

Dylan Glancy¹, Yuwei Zhang², Jamie L Y Wu³, Ben Ouyang³, Seiichi Ohta⁴, Warren C W Chan⁵

Affiliations

¹ Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada; Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada.
² Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada; Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, Toronto, ON M5S 3G9, Canada.
³ Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, Toronto, ON M5S 3G9, Canada.
⁴ Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, Toronto, ON M5S 3G9, Canada; Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo 113-8655, Japan.
⁵ Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada; Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Room 230, Toronto, ON M5S 3E1, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St, Toronto, ON M5S 3G9, Canada; Department of Chemical Engineering, University of Toronto, 200 College Street, Toronto, ON M5S 3E5, Canada; Department of Material Science and Engineering, University of Toronto, 160 College Street, Room 450, Toronto M5S 3E1, Canada. Electronic address: warren.chan@utoronto.ca.

PMID: 31004667
DOI: 10.1016/j.jconrel.2019.04.023

Abstract

Studies into the interactions of serum proteins with nanoparticles are typically performed using nanoparticles that are larger than the size of proteins. Due to this size discrepancy, adsorbed proteins are commonly depicted as a globular structure surrounding a nanoparticle. Here, we asked how we should view nanoparticle-protein complexes when the nanoparticles are of similar size or smaller than the proteins with which they interact. We showed that nanoparticles can serve as a cargo on a protein rather than as a carrier of the protein in a size-dependent manner. This can occur when nanoparticles are below 10 nm in diameter. We discovered that when the nanoparticle is a cargo on the protein, the binding of the protein to the receptor target is minimally affected in contrast to the nanoparticle serving as a carrier. Our study should change how we view and describe nanoparticle-protein complexes when the nanoparticles involved are equal in size or smaller than proteins.

Keywords: Cargo; Carrier; Nanomedicine; Protein corona; Ultrasmall nanoparticles.

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Characterizing the protein corona of sub-10 nm nanoparticles

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