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. 2019 Apr 21;9(4):645.
doi: 10.3390/nano9040645.

Boron Nitride Doped Polyhydroxyalkanoate/Chitosan Nanocomposite for Antibacterial and Biological Applications

Abdul Mukheem  1 , Syed Shahabuddin  2 , Noor Akbar  3 , Azizi Miskon  4 , Norazilawati Muhamad Sarih  5 , Kumar Sudesh  6 , Naveed Ahmed Khan  7 , Rahman Saidur  8   9 , Nanthini Sridewi  10
Affiliations

Boron Nitride Doped Polyhydroxyalkanoate/Chitosan Nanocomposite for Antibacterial and Biological Applications

Abdul Mukheem et al. Nanomaterials (Basel). .

Abstract

The present research focused on the fabrication of biocompatible polyhydroxyalkanoate, chitosan, and hexagonal boron nitride incorporated (PHA/Ch-hBN) nanocomposites through a simple solvent casting technique. The fabricated nanocomposites were comprehensively characterized by Fourier transform infrared spectroscope (FT-IR), field emission scanning electroscope (FESEM), and elemental mapping and thermogravimetric analysis (TGA). The antibacterial activity of nanocomposites were investigated through time-kill method against multi drug resistant (MDR) microbes such as methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli) K1 strains. In addition, nanocomposites have examined for their host cytotoxicity abilities using a Lactate dehydrogenase (LDH) assay against spontaneously immortalized human keratinocytes (HaCaT) cell lines. The results demonstrated highly significant antibacterial activity against MDR organisms and also significant cell viability as compared to the positive control. The fabricated PHA/Ch-hBN nanocomposite demonstrated effective antimicrobial and biocompatibility properties that would feasibly suit antibacterial and biomedical applications.

Keywords: antibacterial; biocompatible; boron nitride; chitosan; nanocomposite; polyhydroxyalkanoate.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic representation of the fabrication of film via solvent casting technique.
Figure 2
Figure 2
Schematic representation of measuring lactate dehydrogenase.
Figure 3
Figure 3
FESEM images of hBN (a), chitosan (c), and a PHA/Ch-hBN nanocomposite (d), as well as a TEM image of hBN (b).
Figure 4
Figure 4
SEM image (a), EDX (g) and mapping (b–f) of PHA/Ch blend doped with 0.1% hBN nanocomposites.
Figure 5
Figure 5
FT-IR (Fourier-transform infrared spectroscopy) spectrum of bare (a) PHA, (b) Chitosan, (c) hBN and, (d) nanocomposites.
Figure 6
Figure 6
TGA thermogram of bare PHA, chitosan, hBN, and PHA/Ch, PHA/Ch-hBN nanocomposites.
Figure 7
Figure 7
(a) Bactericidal activity of nanocomposites against E. coli K1 strain and (b) bactericidal activity against MRSA, shown significant bactericidal efficacy. (Using a two sample T-test, two-tailed distribution, (*) is <0.05; (**) is <0.01; and (***) is <0.001).
Figure 8
Figure 8
Effects of synthesized nanocomposites on the viability of HaCaT cell line, (a–c) represent the negative controls, positive controls, and PHA/Ch-hBN nanocomposite, respectively.
Figure 9
Figure 9
Representative cytotoxic effects of synthesized nanocomposites and positive control (1% Trixton-100) on a HaCaT cell line.
See this image and copyright information in PMC

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