. 2019 Apr 22;17(4):238.

doi: 10.3390/md17040238.

Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines

Andrea Angeli¹, Sonia Del Prete², Sameh M Osman³, Zeid AlOthman⁴, William A Donald⁵, Clemente Capasso⁶, Claudiu T Supuran^{7

8

9}

Affiliations

¹ Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Università degli Studi di Firenze, Via U. Schiff 6, Sesto Fiorentino, 50019 Florence, Italy. andrea.angeli@unifi.it.
² Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy. sonia.delprete@ibbr.cnr.it.
³ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455 Riyadh 11451, Saudi Arabia. smahmoud@ksu.edu.sa.
⁴ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455 Riyadh 11451, Saudi Arabia. zaothman@KSU.EDU.SA.
⁵ School of Chemistry, University of New South Wales, Dalton Building, Sydney, New South Wales 2052, Australia. w.donald@unsw.edu.au.
⁶ Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy. clemente.capasso@ibbr.cnr.it.
⁷ Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Università degli Studi di Firenze, Via U. Schiff 6, Sesto Fiorentino, 50019 Florence, Italy. claudiu.supuran@unifi.it.
⁸ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455 Riyadh 11451, Saudi Arabia. claudiu.supuran@unifi.it.
⁹ School of Chemistry, University of New South Wales, Dalton Building, Sydney, New South Wales 2052, Australia. claudiu.supuran@unifi.it.

PMID: 31013612
PMCID: PMC6520686
DOI: 10.3390/md17040238

Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines

Andrea Angeli et al. Mar Drugs. 2019.

. 2019 Apr 22;17(4):238.

doi: 10.3390/md17040238.

Authors

Andrea Angeli¹, Sonia Del Prete², Sameh M Osman³, Zeid AlOthman⁴, William A Donald⁵, Clemente Capasso⁶, Claudiu T Supuran^{7

8

9}

Affiliations

¹ Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Università degli Studi di Firenze, Via U. Schiff 6, Sesto Fiorentino, 50019 Florence, Italy. andrea.angeli@unifi.it.
² Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy. sonia.delprete@ibbr.cnr.it.
³ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455 Riyadh 11451, Saudi Arabia. smahmoud@ksu.edu.sa.
⁴ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455 Riyadh 11451, Saudi Arabia. zaothman@KSU.EDU.SA.
⁵ School of Chemistry, University of New South Wales, Dalton Building, Sydney, New South Wales 2052, Australia. w.donald@unsw.edu.au.
⁶ Istituto di Bioscienze e Biorisorse, CNR, Via Pietro Castellino 111, 80131 Napoli, Italy. clemente.capasso@ibbr.cnr.it.
⁷ Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Università degli Studi di Firenze, Via U. Schiff 6, Sesto Fiorentino, 50019 Florence, Italy. claudiu.supuran@unifi.it.
⁸ Department of Chemistry, College of Science, King Saud University, P.O. Box 2455 Riyadh 11451, Saudi Arabia. claudiu.supuran@unifi.it.
⁹ School of Chemistry, University of New South Wales, Dalton Building, Sydney, New South Wales 2052, Australia. claudiu.supuran@unifi.it.

PMID: 31013612
PMCID: PMC6520686
DOI: 10.3390/md17040238

Abstract

The γ-carbonic anhydrases (CAs, EC 4.2.1.1) present in the Antarctic marine bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea, herein referred to as PhaCA and CpsCA, respectively, were investigated for their activation with a panel of 24 amino acids and amines. Both bacteria are considered Antarctic models for the investigation of photosynthetic and metabolic pathways in organisms adapted to live in cold seawater. PhaCA was much more sensitive to activation by these compounds compared to the genetically related enzyme CpsCA. The most effective PhaCA activators were d-Phe, l-/d-DOPA, l-Tyr and 2-pyridyl-methylamine, with the activation constant K_A values of 0.72-3.27 µM. d-His, l-Trp, d-Tyr, histamine, dopamine, serotonin anddicarboxylic amino acids were also effective activators of PhaCA, with K_A values of 6.48-9.85 µM. CpsCA was activated by d-Phe, d-DOPA, l-Trp, l-/d-Tyr, 4-amino-l-Phe, histamine, 2-pyridyl-methylamine and l-/d-Glu with K_A values of 11.2-24.4 µM. The most effective CpsCA activator was l-DOPA (K_A of 4.79 µM). Given that modulators of CAs from Antarctic bacteria have not been identified and investigated in detail for their metabolic roles to date, this research sheds some light on these poorly understood processes.

Keywords: Antarctic bacteria; Colwellia psychrerythraea; Pseudoalteromonas haloplanktis; activator; amine; amino acid; carbonic anhydrase; metalloenzymes.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Figure 1**
CA activators CAAs of types 1–24 used in the present study.

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Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines

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Activation Studies of the γ-Carbonic Anhydrases from the Antarctic Marine Bacteria Pseudoalteromonas haloplanktis and Colwellia psychrerythraea with Amino Acids and Amines

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