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. 2019 Mar 20:8:22.
doi: 10.4103/abr.abr_205_18. eCollection 2019.

In vitro Antibacterial Effect of Hydroalcoholic Extract of Lawsonia inermis, Malva sylvestris, and Boswellia serrata on Aggregatibacter actinomycetemcomitans

Surena Vahabi  1 , Mojdeh Hakemi-Vala  2 , Samaneh Gholami  3
Affiliations

In vitro Antibacterial Effect of Hydroalcoholic Extract of Lawsonia inermis, Malva sylvestris, and Boswellia serrata on Aggregatibacter actinomycetemcomitans

Surena Vahabi et al. Adv Biomed Res. .

Erratum in

Abstract

Background: Considering the increased rate of microbial resistance to antibiotics and chemical side effects of antibiotics and antiseptics used for the treatment of periodontal disease, there is a need for an alternative antimicrobial agent with fewer complications. Medicinal herbs have recently become popular as novel antimicrobial agents. This study aimed to assess the antibacterial effects of hydroalcoholic extracts of Lawsonia inermis, Malva sylvestris, and Boswellia serrata on Aggregatibacter actinomycetemcomitans.

Materials and methods: Hydroalcoholic extracts of the three medicinal plants were obtained by the maceration technique and A. actinomycetemcomitans was cultured. Antimicrobial efficacy of the three medicinal plants was compared with that of 0.2% chlorhexidine (CHX) according to the Clinical and Laboratory Standards Institute protocol using agar disc diffusion and broth microdilution techniques. All tests were repeated three times.

Results: Hydroalcoholic extracts of all three plants had antimicrobial activity against A. actinomycetemcomitans. The minimum inhibitory concentration (MIC) of L. inermis, M. sylvestris, and B. serrata was 78.1, 156.2, and 1666 μg/mL with no significant difference between them. The MIC of CHX was 3.33 μg/mL, which was significantly higher than that of B. serrata extract.

Conclusion: Given that further in vivo studies confirm other properties of these extracts and their safety in terms of cytotoxicity and mutagenicity, hydroalcoholic extracts of L. inermis and M. sylvestris may be used in mouthwashes or local delivery systems to affect periodontal biofilm.

Keywords: Aggregatibacter actinomycetemcomitans; Boswellia serrata; Lawsonia inermis; Malva sylvestris; chlorhexidine.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Minimum inhibitory concentration and minimum bactericidal concentration of three herbal essences, chlorhexidine, and doxycycline on Aggregatibacter actinomycetemcomitans in broth microdilution assay following the culture on blood agar well plate
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