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Comparative Study
. 1987 Mar;55(3):579-86.
doi: 10.1128/iai.55.3.579-586.1987.

Purification of Pseudomonas aeruginosa exoenzyme S

D E Woods, J U Que
Comparative Study

Purification of Pseudomonas aeruginosa exoenzyme S

D E Woods et al. Infect Immun. 1987 Mar.

Abstract

Pseudomonas aeruginosa produces two distinct ADP-ribosyl transferases, exotoxin A and exoenzyme S, which differ in a number of properties including substrate specificity. Exoenzyme S was purified from culture supernatants of P. aeruginosa DG1. The procedure for purification consists of four major steps: ammonium sulfate precipitation, anion-exchange chromatography on DEAE-Sephacel, acetone precipitation in the presence of 1 M NaCl, and G-100 Superfine gel filtration chromatography. Exoenzyme S was monitored during purification by an assay for ADP-ribosyl transferase activity, mouse toxicity, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified material exhibited ADP-ribosyl transferase activity, reacted with monoclonal antibodies to exoenzyme S, and was toxic to mice and a variety of tissue culture cell lines.

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