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. 2019 Mar-Apr;10(2):109-117.
doi: 10.32598/bcn.9.10.305. Epub 2019 Mar 1.

Effects of Donepezil Hydrochloride on Neuronal Response of Pyramidal Neurons of the CA1 Hippocampus in Rat Model of Alzheimer's Disease

Azade Eskandary  1 , Ahmad Ali Moazedi  1 , Hosein Najaph Zade  2 , Mohamad Reza Akhond  3
Affiliations

Effects of Donepezil Hydrochloride on Neuronal Response of Pyramidal Neurons of the CA1 Hippocampus in Rat Model of Alzheimer's Disease

Azade Eskandary et al. Basic Clin Neurosci. 2019 Mar-Apr.

Abstract

Introduction: Donepezil (DON), an Acetylcholinesterase Inhibitor (AChEI), is widely used in the treatment of Alzheimer's Disease (AD). The current study aimed at evaluating the effect of donepezil hydrochloride on pyramidal neuron response in CA1 region of a rat model of AD.

Methods: In the current experimental study, adult male Wistar rats were randomly divided into four groups: Nucleus Basalis Magnocellularis (NBM) lesion (the lesions were induced by an electrical method of 0.5 m A, for 3 s in NBM) and three donepezil groups (lesions plus 5, 10, and 15 mg/kg donepezil intraperitoneal injection). Neuronal spontaneous activity to injection of the donepezil and saline were recorded in CA1 region of hippocampal.

Results: The obtained results showed that IntraPeritoneal (IP) injection of donepezil (10 and 15 mg/kg) increased neuronal spontaneous activity in the rat model of AD.

Conclusion: The current study results suggested that acute IP injection of donepezil increased neuronal response in CA1 region of hippocampal in a rat model of AD.

Keywords: Alzheimer’s Disease; Donepezil; Electrophysiology; Rats.

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Conflict of interest statement

Conflict of interest The authors declared no conflict of interest.

Figures

Figure 1.
Figure 1.
Electrical damage site in the NBM
Figure 2.
Figure 2.
Place of electrophysiological recording in CA1 layer
Figure 3.
Figure 3.
Effect of saline on the firing frequency of the CA1 pyramidal neurons
Figure 4.
Figure 4.
Histogram of firing pattern of CA1 pyramidal neurons before and after saline injection
Figure 5.
Figure 5.
Effect of donepezil 5 mg/kg on the firing frequency in CA1 pyramidal neurons (P>0.05)
Figure 6.
Figure 6.
Scatterplot representing the response of pyramidal neurons to saline and donepezil 5 mg/kg injection
Figure 7.
Figure 7.
Effect of donepezil 10 mg/kg on the firing frequency in CA1 pyramidal neurons (P<0.05)
Figure 8.
Figure 8.
The scatterplot representing the response of pyramidal neurons to saline and donepezil 10 mg/kg injection
Figure 9.
Figure 9.
Effect of donepezil 15 mg/kg on firing frequency in CA1 pyramidal neurons (P<0.001)
Figure 10.
Figure 10.
Firing pattern of CA1 pyramidal neuron before and after donepezil 15 mg/kg injection
Figure 11.
Figure 11.
Scatterplot representing the response of pyramidal neurons to saline and donepezil 15 mg/kg injection
See this image and copyright information in PMC

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