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. 1987 Jan;53(1):211-3.
doi: 10.1128/aem.53.1.211-213.1987.

Specific and sensitive plate assay for bacterial lipases

G Kouker, K E Jaeger

Specific and sensitive plate assay for bacterial lipases

G Kouker et al. Appl Environ Microbiol. 1987 Jan.

Abstract

A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat.

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