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. 2019 Apr 30;6(1):12.
doi: 10.1186/s40779-019-0203-z.

Dynamic changes in peripheral blood-targeted miRNA expression profiles in patients with severe traumatic brain injury at high altitude

Si-Qing Ma  1 , Xue-Xia Xu  2 , Zong-Zhao He  2 , Xin-Hui Li  2 , Jun-Ming Luo  3
Affiliations

Dynamic changes in peripheral blood-targeted miRNA expression profiles in patients with severe traumatic brain injury at high altitude

Si-Qing Ma et al. Mil Med Res. .

Abstract

Background: The aim of this work is to detect and compare the peripheral blood miRNA expression profiles in patients with severe traumatic brain injury (sTBI) 2, 12, 24, 48, and 72 h after injury at high altitude and to predict the target genes of differential expressed miRNAs.

Methods: Twenty sTBI patients from high-altitude areas were randomly selected according to the inclusion and exclusion criteria and were divided into five groups: the 2-h group, 12-h group, 24-h group, 48-h group, and 72-h group. Peripheral blood miRNA expression profiles were detected using real-time quantitative PCR (qRT-PCR).

Results: The expression levels of miR-18a, miR-203, miR-146a, miR-149, miR-23b, and miR-let-7b in peripheral blood showed significant differences between the 2-h group and the 12-h group. The expression levels of miR-203, miR-146a, miR-149, miR-23b, and miR-let-7f in peripheral blood were up-regulated in the 24-h group. In the 48-h group, the expression levels of miR-181d, miR-29a, and miR-18b were upregulated. In the 72-h group, the expression levels of miR-203, miR-146a, miR-149, miR-23b, and miR-let-7f changed. The main target genes of the differentiation expressed miRNAs were genes that regulate inflammatory responses, apoptosis, and DNA damage/repair.

Conclusions: miRNAs may be involved in the pathogenesis of sTBI by dynamically regulating the target genes that regulate inflammatory responses, apoptosis, and DNA damage/repair pathways.

Keywords: High altitude; Severe traumatic brain injury; miRNA expression profile.

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Conflict of interest statement

Ethics approval and consent to participate

Written informed consent was obtained from each patient prior to enrollment, and the present study was approved by the ethics committee of Qinghai Province People’s Hospital.

Consent for publication

Not applicable.

Competing interests

All authors have read and approved the final manuscript and declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Changes in miRNA expression profiles in peripheral blood in the 12-h group compared with 2-h group
Fig. 2
Fig. 2
Changes in miRNA expression profiles in peripheral blood in the 24-h group compared with 2-h group
Fig. 3
Fig. 3
Changes in miRNA expression profiles in peripheral blood in the 48-h group compared with 2-h group
Fig. 4
Fig. 4
Changes in miRNA expression profiles in peripheral blood in the 72-h group compared with 2-h group
See this image and copyright information in PMC

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